Vector Laboratories offers a number of reagents that can
be used to detect blotted nucleic acid samples. The label on
the nucleic acid probe determines the choice of reagents.
Biotin - Biotin is one of the most commonly used labels for
nucleic acid probes. Because of the extraordinary affinity of
avidin and streptavidin for biotin and the many biotin-avidin/
streptavidin systems available, this label is ideal for a variety of
applications. The UltraSNAP™ Detection Kit is designed for the
detection of biotinylated nucleic acid probes in Southern blots.
DNP (Dinitrophenyl) - DNP is an excellent alternative to
biotin. A high affinity, purified anti-DNP antibody labeled with
alkaline phosphatase or biotin is available for detection or
amplification of the target.
Fluorescein - Fluorescein can be directly visualized (ex 495 nm;
em 515 nm) but is generally detected using a biotin or enzyme
conjugated, affinity-purified, anti-fluorescein antibody.
Texas Red® - Texas Red®, a rhodamine fluorochrome, has a
high quantum yield (ex 595 nm; em 615 nm), but generally
is not visualized directly. The label is usually detected using
a high affinity, alkaline phosphatase or biotin-conjugated
antibody to rhodamine.
|
|