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Recognized as a leader in the development of detection systems, Vector Laboratories has been providing the research community with quality reagents yielding high sensitivity and low background since 1976. Continuing in this tradition, our molecular biology tools offer sensitivity, reliability, simplicity, and economy. These systems and reagents facilitate the labeling, detection, and manipulation of nucleic acids. Microarrays, blotting assays, cell trafficking, in situ hybridization, comparative genomic hybridization, subtractive hybridization, and affinity purification are just a few applications in which our molecular biology tools can be successfully employed.
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A free, full color booklet entitled Guide to Nucleic Acid Labeling and Detection Systems is available from Vector Laboratories. Ideal for those new to the field or those requiring novel, cost effective approaches in optimal labeling and detection of DNA/RNA probes. Instructive diagrams showing labeling methodologies are accompanied by photographic examples submitted by independent investigators that highlights their latest research. Practical insight is offered into choice of labeling method based on probe size, type, application and tag. Subsequent detection of the labeled probes using chromogenic or fluorescent visualization is described for various applications including microarrays, blotting and in situ hybridization. Step-by-step protocols and referenced publications make this a valuable and contemporary reference tool. Preview a copy here.
Please download here or request a free copy on our Catalog & Brochures request page under the Support menu.
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MBB.pdf (PDF)
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Labeling of Nucleic Acids
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The PHOTOPROBE® reagents, FastTag® Systems, and the 3’ and 5’ EndTag™ Labeling Systems are efficient and reliable labeling methods that offer choices for optimum labeling for a given application, as well as choices for incorporating fluorochromes, haptens, or affinity tags.
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Nucleic acid probes for applications such as in situ hybridization, northern and Southern blot hybridization, comparative genomic hybridization, intracellular localization experiments, and DNA microarrays can be easily and optimally labeled and subsequently visualized with our comprehensive range of fluorescent, chemiluminescent, or chromogenic detection systems.
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Detection of Nucleic Acid Labels
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Each choice of label is complemented by several different fluorescent or enzyme-based detection reagents providing the researcher with flexibility in optimizing experimental systems. Among the extensive range of antibody conjugates and avidin/streptavidin conjugates, amplification reagents such as Biotinylated Anti-Streptavidin and Biotinylated Anti-Avidin are also available. These reagents reliably increase sensitivities of target signals in microarray applications and fluorescence in situ hybridization. In addition, the superior antifading property of the VECTASHIELD® line of mounting media ensures the preservation of signal intensity in applications such as fluorescence in situ hybridization.
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For nucleic acid blotting applications, the Vector® UltraSNAP™ Detection System containing specially formulated reagents detects biotinylated probes with high sensitivity and low background. This system utilizes the DuoLuX™ Chemiluminescent/Fluorescent Substrate with intense and prolonged light emission characteristics, ideal for Southern and northern hybridization, dot blot, plaque or colony screening.
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Immobilization of Nucleic Acids
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Vector Laboratories offers both irreversible (VECTREX® Avidin D) as well as reversible (VECTREX® Avidin DLA or VECTREX® AAL) affinity binding matrices. Applications like genomic/ cDNA subtraction or library screening by hybrid capture require labeled nucleic acids to be immobilized onto a solid support. Using our affinity binding matrices in such applications can yield information about differential gene expression or allow the isolation and subsequent amplification of a gene of interest.
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Electrophoretic Resolution of Similarly Sized DNA in a Sequence Specific Manner
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The Resolve-It™ Kit contains two sequence-specific DNA ligands which bind to DNA and retard its electrophoretic migration in a sequence specific manner. The separation of the DNA species allows for the gel purification of DNA samples which would otherwise be very difficult to isolate. The ability to resolve DNA in a gel is critical for applications such as differential display in which multiple DNA species of similar size need to be separated before subsequent excision and analysis.
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Quantitation of Biotin using the Quant*Tag™ System
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The Quant*Tag™ Biotin Kit is designed to determine the amount of free biotin in solution or the number of biotins attached to proteins, nucleic acids or other macromolecules. Samples do not need to be predigested. The kit reagents chemically react with free or bound biotin, producing a colored product that can be quantified using a spectrophotometer. The absorbance is measured in the visible spectrum, allowing the use of plastic cuvettes or microtitre plates.
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