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The ProtOn™ protein labeling kits are designed for the simple and fast labeling of proteins with biotin or fluorescein. The kits are ideally suited to label antibodies, hormones, or other proteins as well as other macromolecules containing primary amino groups. Labeled proteins can be used in a number of applications, such as immunological assays, fluorescence microscopy, investigation of protein-protein interactions, etc.
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Each kit contains all the components needed for at least five labeling reactions (up to 1 mg of protein per reaction). Kit components include:
Labeling Reagent*
Reagent Solvent
Reaction Buffer
Stop Reagent
Gel Filtration Slurry
5 x 1 ml Spin Columns
5 x 2 ml Collection Tubes
Agarose Avidin D Slurry**
*N-hydroxysuccinimidyl-6-(biotin amido) hexanoate or fluorescein isothiocyanate.
**Included in biotin labeling kit only (for measuring degree of biotinylation).
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The ProtOn™ labeling reagents react with primary amines (i.e. lysine residues or terminal amino groups) on the protein, forming a covalent bond. The linkage between the label and the protein produced with these reagents is stable, with no significant loss of the label during storage.
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Each ProtOn™ Kit is supplied with complete instructions that offer the researcher a step-by-step guide to convenient and easy protein labeling with biotin or fluorescein.
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Paraffin embedded human prostate tissue stained using rabbit anti-PSA labeled with the ProtOn™ Biotin Labeling Kit and detected with VECTASTAIN® Elite® ABC Reagent and Vector® DAB substrate.
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Paraffin embedded human prostate tissue stained directly with rabbit anti-PSA labeled with the ProtOn™ Fluorescein Labeling Kit and mounted in VECTASHIELD® HardSet™ and PI.
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Thiol-Reactive Labeling Reagents
Thiol-reactive labeling reagents are designed for labeling of proteins, nucleic acids or other molecules containing one or more thiol groups. Molecules to be labeled with a maleimide labeling reagent require free thiol groups. In the case of proteins, the maleimide will react with exposed cysteine residues. Alternatively, free thiols may be generated by reducing disulfide bonds or by modifying other reactive groups such as primary amines with compounds like Traut's reagent (2-iminothiolane).
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