Application: Nucleic Acid Detection
      

The DuoLuX™ Chemiluminescent/Chemifluorescent Substrate is ideal for development of protein and nucleic acid blots. Complete kits are available for western and Southern/northern blot development (VECTASTAIN® ABC-AmP™ system and Vector® UltraSNAP™ Kit, respectively.) For developing Southern, northern, or dot blots using alkaline phosphatase, typical film exposure times range from 30 seconds to 10 minutes with sensitivity ranging from 100 fg to 10 pg of nucleic acid. Using peroxidase, exposure times are somewhat shorter and sensitivities are slightly less than those for alkaline phosphatase. Both nitrocellulose or nylon membranes can be used. The UltraSNAP™ Detection System for Nucleic Acid Blots contains the DuoLuX™ Substrate and specially formulated essential reagents to provide optimal detection of biotin labeled nucleic acids on blots using an alkaline phosphatase system.

Southern blot of the E. coli phosphoenolpyruvate carboxylase gene. Various dilutions of a 1.3 kb PCR product of the E. coli ppc gene was labeled with PHOTOPROBE® (LA) Biotin and hybridized to immobilized Tsp509 I-digested E. coli genomic DNA. Probe was detected using Alkaline Phosphatase Streptavidin followed by DuoLuX™ Substrate visualized by chemiluminescence.