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Featuring:
Plus
quality reagents for:
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| Mouse on Mouse (M.O.M.) Basic Kit
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Section of mouse intestine double stained with mouse antibodies against peripherin and desmin, each detected with Vector MOM Basic Kit, using Fluorescein Avidin DCS and Texas Red Avidin DCS, respecitvely.
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The Vector® M.O.M.™ Immunodetection Kits are specifically
designed to localize mouse primary antibodies on mouse
tissues. The major problem with using mouse primary
antibodies on mouse tissues in immunohistochemistry is the
inability of the anti-mouse secondary antibody to distinguish
between the mouse primary antibody and endogenous
mouse immunoglobulins in the tissue. This can result in high
background staining which obscures specific staining. This
problem can be essentially eliminated by using a Vector®
M.O.M.™ Immunodetection Kit which includes a proprietary
M.O.M.™ Mouse Ig Blocking Reagent with a specialized
M.O.M.™ Biotinylated Anti-Mouse Ig to significantly reduce
undesired binding of the secondary antibody to endogenous
tissue immunoglobulin.
Key advantages of the Vector® M.O.M.™
Immunodetection Kits:
Significant reduction of endogenous mouse Ig staining when
using mouse primary antibodies on mouse tissue
Clear, crisp specific staining of antigens of interest
Based on VECTASTAIN® ABC reagent protocols
Procedures are simple and easy to follow
No tedious calculations or antibody prebinding steps
required
The Vector® M.O.M.™ Immunodetection Kits are useful in any
mouse-on-mouse application, such as studies in genetically
engineered mice, including transgenic and knock-out models,
as well as mouse xenografts.
The M.O.M.™ Kits can be also used in multiple antigen staining
protocols to localize two different mouse primary antibodies on
the same mouse tissue sections using fluorescent or enzyme-based
detection systems. Detailed information for multiple
antigen labeling can be found in our brochure, “ Discovery
Through Color: A Guide to Multiple Antigen Labeling”,
available on our website.
Three Vector® M.O.M.™ Kits are available. All kits use the
same proprietary M.O.M.™ Mouse Ig Blocking Reagent and
the M.O.M.™ Biotinylated Anti-Mouse Ig Reagent, but offer a
choice of an enzyme-based or fluorescent-based visualization
method. M.O.M.™ Mouse Ig Blocking Reagent and M.O.M.™
Biotinylated Anti-Mouse Ig Reagent are also available
separately.
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Vector® M.O.M.™ Basic Kit (BMK-2202) includes the
following:
M.O.M.™ Mouse Ig Blocking Reagent
M.O.M.™ Biotinylated Anti-Mouse Ig Reagent
M.O.M.™ Protein Concentrate for general protein blocking
and diluent
An enzyme or fluorochrome conjugate is not included allowing
for a choice of any avidin or streptavidin-based detection
system. Choose any peroxidase or alkaline phosphatase
conjugated avidin or streptavidin for chromogenic detection
(for example, VECTASTAIN® ABC-AP Standard Kit followed
by a Vector® alkaline phosphatase substrate), or choose a
fluorochrome-labeled avidin or streptavidin.
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This Vector® M.O.M.™ kit contains sufficient stock reagents to provide approximately 25 ml of working solution, which is usually enough to stain about 125-250 mouse tissue sections.
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Sections of mouse kidney stained with mouse antibody against smooth muscle actin using VECTASTAIN® ABC-AP reagents and Vector® Blue substrate. Left photo: Using standard biotinylated anti-mouse antibody and normal blocking serum, confusing background interference is seen. Right photo: With the Vector® M.O.M.™ Basic kit, clean background and specific staining is achieved.
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In addition, the Vector® M.O.M.™ Basic kit can be used to localize two different mouse primary antibodies in the same mouse tissue section. By using a multiple antigen staining protocol, two fluorescent avidin labels or two enzyme-based stains can be introduced on one section.
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Product review on Biocompare.com.
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