VECTASTAIN Universal Quick Kit (concentrate)


Prostate: Desmin (m), VECTASTAIN Universal Quick Kit, ImmPACT DAB (brown) substrate. Hematoxylin QS (blue) counterstain.
 

Catalog # Unit Size Product Information MSDS
PK-8800    1 kit   Multiple Antigen Labeling Guide
Protocol
Protocol (CE)
Enzyme Immunoassays, Hybridoma Screening, and Western Blots
Troubleshooting Guide
Enzyme Substrate Combinations Chart
Quenching Endogenous Peroxidase Activity  
PK-8800eumsds.pdf  


With more than 40,000 citations to its credit, the VECTASTAIN® ABC kit remains widely popular. Based on the versatile biotin/avidin interaction, the system is modular, and along with our selection of secondary antibodies, can accommodate a wide array of primary antibody and tissue species. Our ABC kits are economical and continue to be a staple product in any immunohistochemistry laboratory.

Peroxidase substrates produce sharp, dense precipitates with crisp localization. These characteristics, in conjuction with the high sensitivity and low background of the VECTASTAIN® ABC systems, make the peroxidase enzyme a preferred choice in many applications. (eg. In neural tissue, the peroxidase system is often preferred because it gives more consistent labeling of both cell bodies and processes.)

VECTASTAIN® Universal Quick Kits

The VECTASTAIN® Universal Quick Kit is designed for rapid immunohistochemical staining of tissue sections. The kit relies on a proprietary preformed peroxidase-streptavidin complex to achieve outstanding sensitivity with short incubation times.

  • Rapid protocol: Staining in less than 20 minutes following primary antibody incubation. Working solutions can be used immediately after dilution.

  • High sensitivity, low background.

  • Biotinylated, Universal Pan-Specific secondary antibody recognizes mouse, rabbit, and goat primary antibodies as well as those from related species such as rat, bovine, and sheep.*

  • Available in Ready-To-Use (R.T.U) format: Prediluted, stabilized working solutions of VECTASTAIN® Universal Quick Kit reagents provide the same high sensitivity and low background as the concentrated VECTASTAIN® Universal Kit.

    * Do not use the pan-specific secondary antibody to stain rat, mouse or other rodent, rabbit, goat, bovine, or sheep tissue due to potential reactivity with endogenous IgG.
     
  • Kit Contents:

  • 6 ml normal horse blocking serum
  • 2.2 ml biotinylated universal, pan-specific (anti-mouse/rabbit/goat IgG) antibody
  • 1.2 ml streptavidin peroxidase preformed complex

    Sufficient reagents to stain approximately 250-500 sections.

    For additional information on the VECTASTAIN® ABC systems, please click on the link below.
     
  • VECTASTAIN ABC Systems  
     
     

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