VECTASTAIN Universal Quick Kit, R.T.U. (Ready-to-Use)


Human Breast Carcinoma: Ki67 (m), VECTASTAIN Universal Quick Kit with DAB (brown) substrate. Hematoxylin (blue) counterstain.
 

Catalog # Unit Size Product Information MSDS
PK-7800    50 ml   Multiple Antigen Labeling Guide
Protocol
Enzyme Immunoassays, Hybridoma Screening, and Western Blots
Troubleshooting Guide
Quenching Endogenous Peroxidase Activity
Enzyme Substrate Combinations Chart  
msdsPK7800.pdf  


With more than 40,000 citations to its credit, the VECTASTAIN® ABC kit remains widely popular. Based on the versatile biotin/avidin interaction, the system is modular, and along with our selection of secondary antibodies, can accommodate a wide array of primary antibody and tissue species. Our ABC kits are economical and continue to be a staple product in any immunohistochemistry laboratory.

Peroxidase substrates produce sharp, dense precipitates with crisp localization. These characteristics, in conjuction with the high sensitivity and low background of the VECTASTAIN® ABC systems, make the peroxidase enzyme a preferred choice in many applications. (eg. In neural tissue, the peroxidase system is often preferred because it gives more consistent labeling of both cell bodies and processes.)

VECTASTAIN® Universal Quick Kits

The VECTASTAIN® Universal Quick Kit is designed for rapid immunohistochemical staining of tissue sections. The kit relies on a proprietary preformed peroxidase-streptavidin complex to achieve outstanding sensitivity with short incubation times.

  • Rapid protocol: Staining in less than 20 minutes following primary antibody incubation. Working solutions can be used immediately after dilution.

  • High sensitivity, low background.

  • Biotinylated, Universal Pan-Specific secondary antibody recognizes mouse, rabbit, and goat primary antibodies as well as those from related species such as rat, bovine, and sheep.*

  • Available in Ready-To-Use (R.T.U) format: Prediluted, stabilized working solutions of VECTASTAIN® Universal Quick Kit reagents provide the same high sensitivity and low background as the concentrated VECTASTAIN® Universal Kit.

    * Do not use the pan-specific secondary antibody to stain rat, mouse or other rodent, rabbit, goat, bovine, or sheep tissue due to potential reactivity with endogenous IgG.
     
  • Kit Contents:

    Each of the stabilized, prediluted reagents are supplied in dropper bottles.

  • 50 ml 2.5% normal horse serum
  • 50 ml prediluted, biotinylated, universal pan-specific (anti-mouse/rabbit/goat IgG) secondary antibody
  • 50 ml prediluted peroxidase-streptavidin complex

    Sufficient reagents to stain approximately 250-500 sections.

    For additional information on the VECTASTAIN® ABC systems, please click on the link below.
     
  • VECTASTAIN ABC Systems  
     
     

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