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	ProtOn Biotin Protein Labeling Kit

Paraffin embedded human prostate tissue stained using rabbit anti-prostate specific antigen labeled with the ProtOn Biotin Labeling Kit and detected with VECTASTAIN Elite ABC Reagent and Vector DAB substrate.

Catalog #	Unit Size	Product Information	MSDS
PLK-1202	1 kit	References Protocol	msdsPLK1202.pdf

Protein labeling and modification is a powerful tool for the isolation and analysis of proteins. The ProtOn™ Protein Labeling Kits simplify the attachment of either biotin or fluorescein to antibodies or other proteins. Vector Laboratories offers a variety of reagents and kits for protein labeling allowing for greater flexibility of experimental design.

This ProtOn™ protein labeling kit is designed for the simple and fast labeling of proteins with biotin. The kit is ideally suited to label antibodies, hormones, or other proteins as well as other macromolecules containing primary amino groups. Labeled proteins can be used in a number of applications, such as immunological assays, fluorescence microscopy, investigation of protein-protein interactions, etc.

The ProtOn™ labeling reagent reacts with primary amines (i.e. lysine residues or terminal amino groups) on the protein forming a stable covalent bond. Each kit contains all the components needed for at least five labeling reactions (up to 1 mg of protein per reaction):

Labeling Reagent (Biotin [Long Arm] NHS)

Reagent Solvent

Reaction Buffer

Stop Reagent

Gel Filtration Slurry

5 x 1 ml Spin Columns

5 x 2 ml Collection Tubes

Agarose Avidin D Slurry**

**Included in biotin labeling kit only (for measuring degree of biotinylation).

ProtOn Biotin Labeling Kit