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Featuring:
Plus
quality reagents for:
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| Streptavidin, Phycoerythrin conjugated (SAPE)
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Vector Laboratories’ fluorochrome-conjugated streptavidin and
avidin reagents are highly purified and possess very low non-specific
binding properties. They have extremely high affinity
for biotin. These fluorescent conjugates can be used to detect
biotinylated secondary antibodies and other macromolecules in
applications such as immunofluorescence, in situ hybridization,
or flow cytometry.
Amplification of fluorescent signals can be easily achieved with
our biotinylated secondary antibodies followed by our highly
purified fluorochrome-labeled streptavidin or avidin. Using a
biotin/avidin or biotin/streptavidin detection system results in
an additional layer of amplification over a directly conjugated
secondary antibody.
Phycoerythrin is a protein that produces a bright red-orange
fluorescence. Our newly improved Phycoerythrin Streptavidin
(SA-5207) is substantially brighter than previous conjugates.
Phycoerythrin Streptavidin can be used on gene chips, in cell
sorting, and in tissue staining.
Phycoerythrin Streptavidin has a broad absorption range from 450 nm to 580 nm and a maximum emission at about
570-590 nm. It is a highly fluorescent label which can overcome problems of endogenous fluorescence.
If this conjugate is used in tissue staining, VECTASHIELD HardSet Mounting Medium is recommended for mounting. Optimal signal characteristics are maintained for 24 hours following mounting.
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