VECTASTAIN ABC Kit (Mouse IgG )


Tumor (double label): Cytokeratin, VECTASTAIN Elite ABC Kit, Vector NovaRED (red) substrate. CD34, VECTASTAIN ABC-AP Kit, BCIP/NBT (blue) substrate.
 

Catalog # Unit Size Product Information MSDS
PK-4002    1 kit   Enzyme Immunoassays, Hybridoma Screening, and Western Blots
Troubleshooting Guide
Enzyme Substrate Combinations Chart
Multiple Antigen Labeling Guide
Protocol
Quenching Endogenous Peroxidase Activity  
msdsPK4000 series.pdf  


With more than 40,000 citations to its credit, the VECTASTAIN® ABC kit remains widely popular. Based on the versatile biotin/avidin interaction, the system is modular, and along with our selection of secondary antibodies, can accommodate a wide array of primary antibody and tissue species. Our ABC kits are economical and continue to be a staple product in any immunohistochemistry laboratory.

Peroxidase substrates produce sharp, dense precipitates with crisp localization. These characteristics, in conjuction with the high sensitivity and low background of the VECTASTAIN® ABC systems, make the peroxidase enzyme a preferred choice in many applications. (eg. In neural tissue, the peroxidase system is often preferred because it gives more consistent labeling of both cell bodies and processes.)

Original VECTASTAIN® ABC Kit

  • Good sensitivity/low background
  • Original avidin/biotin ABC complex formulation
  • Low cost
  • Available without (“Standard” kit) or with biotinylated species-specific secondary antibody.

    Kit Contents:

  • 3 ml normal horse serum
  • 1 ml biotinylated horse anti-mouse IgG secondary antibody
  • 2 ml each of concentrated A and B reagents

    Sufficient reagents to stain approximately 1000-2000 sections.

    Product review on Biocompare.com.

    For additional information on the VECTASTAIN® ABC systems, please click on the link below.
     
  • VECTASTAIN ABC Systems  
     
     

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