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	VECTASTAIN Elite ABC Kit (Universal)

Tonsil (triple label): CD3 (m), VECTASTAIN Universal Elite ABC Kit, DAB (brown); CD20 (m), VECTASTAIN Universal Elite ABC Kit, Vector VIP substrate (purple); Multi-cytokeratin (m), VECTASTAIN Universal Elite ABC Kit, Vector SG substrate (blue/gray).

Catalog #	Unit Size	Product Information	MSDS
PK-6200	1 kit	Multiple Antigen Labeling Guide Protocol (CE) Protocol Enzyme Immunoassays, Hybridoma Screening, and Western Blots Troubleshooting Guide Enzyme Substrate Combinations Chart Quenching Endogenous Peroxidase Activity	PK-6100series.eumsds.pdf

VECTASTAIN Elite ABC Video Tutorial

Tissue staining using the VECTASTAIN Elite ABC system. For additional instructional videos please see the Tutorials page under the Support link.

With more than 40,000 citations to its credit, the VECTASTAIN^® ABC kit remains widely popular. Based on the versatile biotin/avidin interaction, the system is modular, and along with our selection of secondary antibodies, can accommodate a wide array of primary antibody and tissue species. Our ABC kits are economical and continue to be a staple product in any immunohistochemistry laboratory.

Peroxidase substrates produce sharp, dense precipitates with crisp localization. These characteristics, in conjuction with the high sensitivity and low background of the VECTASTAIN^® ABC systems, make the peroxidase enzyme a preferred choice in many applications. (eg. In neural tissue, the peroxidase system is often preferred because it gives more consistent labeling of both cell bodies and processes.)

VECTASTAIN^® Elite_® ABC System

Advanced avidin/biotin technology: The Elite_® ABC complex is smaller, very uniform, and highly active, allowing more accessibility for binding to a biotinylated target.

Highest sensitivity, low background: The VECTASTAIN^® Elite_® ABC system is the most sensitive avidin/biotin-based peroxidase system. The Elite_® ABC series is approximately 5 times more sensitive than the original VECTASTAIN^® ABC Kit with the same low background.

Cost effective: Higher sensitivity leads to lower cost per slide.

Available without (“Standard” kit) or with biotinylated species specific or universal secondary antibodies.

Available in Ready-To-Use (R.T.U.) formats: Prediluted, stabilized working solutions of Elite_® ABC Kit reagents provide the same high sensitivity and low background as the traditional VECTASTAIN^® Elite_® ABC Kit reagents.

Kit Contents:

3 ml normal horse serum

2 ml concentrated universal biotinylated anti-mouse/rabbit IgG secondary antibody

2 ml each of concentrated A and B reagents.

Sufficient reagents to stain approximately 500-1000 sections.

Product review on Biocompare.com.

Note: Our Universal Biotinylated Anti-Mouse/Rabbit IgG secondary antibody included in this kit (Cat. No. BA-1400) is a cocktail of biotinylated anti-mouse IgG and anti-rabbit IgG, designed for use with both rabbit and mouse primary antibodies. This antibody should not be used to stain tissues from rodents or rabbits due to its reactivity with IgG present in the tissues of these species.

For additional information on the VECTASTAIN^® ABC systems, please click on the link below.

VECTASTAIN ABC Systems