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Featuring:
Plus
quality reagents for:
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| Agarose Avidin D
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Agarose Avidin D (A-2010) is prepared by conjugating
Avidin D to heat stable, cross-linked 4% agarose gel beads.
To ensure minimal steric interference and low nonspecific
binding, Avidin D is conjugated through a hydrophilic spacer
arm. The procedure we have developed for coupling Avidin D
to agarose optimally preserves the biotin binding activity of the
Avidin D. Unlike cyanogen bromide coupling, our procedure
does not produce conjugates which can be leached from the
gel with solutes such as Tris buffer. Our procedure also does
not generate charged groups on the gel that can bind proteins
nonspecifically. Protein concentration is 2 mg Avidin D per
ml of settled agarose beads. The product is supplied as a 1:1
suspension in buffer. The binding capacity is greater than 2 mg
of biotinylated IgG per ml of settled beads.
This product can be used to separate biotinylated
macromolecules from unbiotinylated materials or for solid phase
binding assays.
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