Protocols  Selected Vector kit instructions and associated protocols are listed below in PDF format. These files can be opened using the Adobe Acrobat Reader 3.0 or higher. If you are unable to obtain the desired information please contact Vector technical service or your local Vector distributor for further assistance.  VECTASTAIN® Kits VECTASTAIN® Elite ABC-Peroxidase Kit (included in all PK-6100 series kits) VECTASTAIN® Universal Elite ABC-Peroxidase Kit (PK-6200) R.T.U. VECTASTAIN® Elite ABC-Peroxidase Reagent (PK-7100) R.T.U. VECTASTAIN® Universal Elite ABC-Peroxidase Kit (PK-7200) VECTASTAIN® ABC-Peroxidase Kit (included in all PK-4000 series kits) VECTASTAIN® ABC-Alkaline Phosphatase Kit (included in all AK-5000 series kits) VECTASTAIN® Universal ABC-Alkaline Phosphatase Kit (AK-5200) VECTASTAIN® ABC-GO-Glucose Oxidase Kit (included in all OK-3000 series kits) VECTASTAIN® Universal Quick Kit (PK-8800) R.T.U. VECTASTAIN® Universal Quick Kit (PK-7800) Vector Mouse on Mouse (M.O.M.™) Immunodetection Kit Peroxidase Vector® Mouse on Mouse (M.O.M.™) Immunodetection Kit (PK-2200) Fluorescein Vector® Mouse on Mouse (M.O.M.™) Immunodetection Kit (FMK-2201) Basic Vector® Mouse on Mouse (M.O.M.™) Immunodectection Kit (BMK-2202) Counterstains Vector® Hematoxylin (H-3401) Vector® Hematoxylin QS (H-3404) Vector® Methyl Green (H-3402) Vector® Nuclear Fast Red (H-3403) Counterstain/Substrate Compatibility Chart Mounting Media VectaMount™Permanent Mounting Medium (H-5000) VECTASHIELD® Mounting Media (H-1000) VECTASHIELD® Mounting Media with DAPI (H-1200) VECTASHIELD® Mounting Media with PI (H-1300) VECTASHIELD® HardSet Mounting Media (H-1400) VECTASHIELD® HardSet Mounting Media with DAPI (H-1500) VECTABOND™ Reagent for Tissue Section Adhesion (SP-1800) Antigen Unmasking Solution (H-3300) Avidin/Biotin Blocking Kit (SP-2001) Streptavidin/Biotin Blocking Kit (SP-2002) Enzyme Substrates Peroxidase DAB Substrate Kit (SK-4100) DAB Enhancing Solution (H-2200) Vector® VIP Substrate Kit (SK-4600) Vector® SG Substrate Kit (SK-4700) Vector® NovaRED Substrate Kit (SK-4800) AEC Substrate Kit (SK4200) TMB Substrate Kit (SK-4400) 4-CN Substrate Kit (SK-4300) ABTS Substrate Kit (SK-4500) DuoLuX™ Chemiluminescent/Fluorescent Substrate Kit for peroxidase (SK-6604) Alkaline Phosphatase Vector® Red Substrate Kit (SK-5100) Vector® Blue Substrate Kit (SK-5300) Vector® Black Substrate Kit (SK-5200) BCIP/NBT Substrate Kit (SK-5400) Levamisole Solution (SP-5000) p-Nitrophenyl phosphate Substrate Kit (SK-5900) DuoLuX™ Chemiluminescent/Fluorescent Substrate Kit for alkaline phosphatase (SK-6605) Glucose Oxidase Glucose Oxidase Substrate Kit I (SK-3100) Glucose Oxidase Substrate Kit II (SK-3200) Glucose Oxidase Substrate Kit III (SK-3300) Chemiluminescent/Fluorescent Substrate DuoLuX™ Chemiluminescent/Fluorescent Substrate Kit for peroxidase (SK-6604) DuoLuX™ Chemiluminescent/Fluorescent Substrate Kit for alkaline phosphatase (SK-6605) Blot Detection Systems VECTASTAIN® ABC-AmP™ Western Blot Detection System (AK-6000 series kits) Vector® UltraSNAP™ Detection System for nucleic acid blots (MB-6500) In Situ Hybridization Fluorescence Detection of Biotin-Labeled ISH Probes Fluorescence Detection of Fluorescein-Labeled ISH Probes Chromogenic Detection of Biotin-Labeled ISH Probes Chromogenic Detection of Fluorescein-Labeled ISH Probes In Situ Hybridization Detection Systems (General guidelines) NicKit™ Probe Size Optimization Kit (MB-1905) Amplification of FISH using Biotinylated Anti-Avidin (BA-0300) Protein Labeling Kits ProtOn™ Biotin Labeling Kit ProtOn™ Fluorescein Labeling Kit Biotin Labeling Reagents Biotin (Long Arm) NHS (SP-1200) Biotin (Long Arm) NHS, water soluble (SP-1210) Biotin (Long Arm) Maleimide (SP-1501) Biotin (Long Arm) Hydrazide (SP-1100) Thiol-Reactive Labeling Reagents Biotin Maleimide (SP-1501) Coumarin 151 Maleimide (SP-1506) Dinitrophenyl Maleimide (SP-1503) Fluorescein Maleimide (SP-1502) Fucose Maleimide (SP-1504) Texas Red Maleimide (SP-1505) Molecular Biology Reagents 5' EndTag Labeling System (MB-9001) 3' EndTag Labeling System (MB-9002) PHOTOPROBE® Biotin (SP-1000, SP-1010, SP-1020, SPK-1906) FastTag® Biotin (included in all MB-2000 series kits) FastTag® Dinitrophenyl (DNP; included in all MB-3000 series kits) FastTag® Fluorescein (FL; included in all MB-1000 series kits) FastTag® Fucose (included in all MB-4000 series kits) FastTag® Texas Red (TR; included in all MB-5000 series kits) FastTag® Basic Kit (MB-8000) NicKit™ Probe Size Optimization Kit (MB-1905) In Situ Hybridization Detection Systems Amplification of FISH using Biotinylated Anti-Avidin (BA-0300) Detection of Labeled Nucleic Acid Probes on Blots Spin Column Chromatography Modified FastTag® labeling protocol for PCR Primers Irreversible Affinity Binding Matrices VECTREX® Avidin D (A-2020) Reversible Affinity Binding Matrices VECTREX® Avidin DLA (MB-2022) VECTREX® AAL (MB-1397) Fusion Protein Reagents Vector Fusion-Aid - MBP Kit (MB-0731) Vector Fusion-Aid - GFP Kit (MB-0732) Vector Fusion-Aid - C-MYC Kit (MB-0733) Vector Fusion-Aid - HA Kit (MB-0734) Neuronal Tracing reagents PHA-L (L-1110) NEUROBIOTIN™ Tracer (SP-1120) Lectins Peroxidase localization of WGA in Neural Tissue (L-1020) Enrichment of NK cells by panning on ECL plates (L-1140) Specificity of Jacalin binding (L-1150) Supplemental Protocols Enzyme Immunoassays, Hybridoma Screening, and Western Blots Quenching of Endogenous Peroxidase Activity Recommended Dilutions and Buffers Troubleshooting Guide Troubleshooting mouse primaries on mouse tissue Enzyme Substrate Compatibility Chart Double Immunofluorescent Labeling