Tutorials

The following video tutorials are step by step demonstrations of various procedures in immunohistochemistry and molecular biology. Please select a video tutorial from the menu below.

Immunohistochemical Staining Protocol
Deparaffinization - Removal of wax and rehydration of tissue sections
Antigen Retrieval - Reliable and consistent pressure cooker technique
Blocking Endogenous Peroxidase Activity - Use of hydrogen peroxide

Tissue Staining

• Using VECTASTAIN ABC system - Procedure for avidin-biotin enzyme complex
• Using ImmPRESS Reagents - Procedure using non-biotin enzyme polymer system

Peroxidase Substrate

• ImmPACT DAB
• Vector DAB
• Vector NovaRED
• Vector VIP
• Vector SG
• AEC

Permanent (non-aqueous) Mounting - Dehydration, mounting and coverslipping using Vectamount Mounting Medium

Aqueous Mounting - Vectamount AQ Mounting Medium

Nucleic Acid Labeling

• Nucleic Acid Labeling Guide - Interactive; helps you choose a labeling system
• FastTag System - Labeling nucleic acids with haptens, fluorochromes, or ligands
• PHOTOPROBE Biotin System - Labeling DNA with biotin using heat or light.

Dot Blot Assay - Dotting and detecting labeled probes on a membrane

Protein Labeling

• Fluorescein - Labeling proteins with fluorescein
• Biotin - Labeling proteins with biotin

Biotin Quantitation - Sensitive method of measuring biotin, free or bound to macromolecules using the QuantTag Kit.

Independent Tutorials

Immunohistochemistry using the ABC system

Western Blotting using the ABC system