With more than 40,000
citations to its credit, the VECTASTAIN® ABC kit remains widely
popular. Based on the versatile biotin/avidin interaction, the
system is modular, and along with our selection of secondary
antibodies, can accommodate a wide array of primary antibody
and tissue species. Our ABC kits are economical and continue
to be a staple product in any immunohistochemistry laboratory.
Peroxidase substrates produce sharp, dense precipitates with
crisp localization. These characteristics, in conjuction with the
high sensitivity and low background of the VECTASTAIN® ABC
systems, make the peroxidase enzyme a preferred choice
in many applications. (eg. In neural tissue, the peroxidase
system is often preferred because it gives more consistent
labeling of both cell bodies and processes.)
VECTASTAIN® Elite® ABC System
Advanced avidin/biotin technology: The Elite® ABC complex
is smaller, very uniform, and highly active, allowing more
accessibility for binding to a biotinylated target.
Highest sensitivity, low background: The VECTASTAIN®
Elite® ABC system is the most sensitive avidin/biotin-based
peroxidase system. The Elite® ABC series is approximately 5
times more sensitive than the original VECTASTAIN® ABC Kit
with the same low background.
Cost effective: Higher sensitivity leads to lower cost per
slide.
Available without (“Standard” kit) or with biotinylated species specific
or universal secondary antibodies.
Available in Ready-To-Use (R.T.U.) formats: Prediluted,
stabilized working solutions of Elite® ABC Kit reagents
provide the same high sensitivity and low background as
the traditional VECTASTAIN® Elite® ABC Kit reagents.
Product review on Biocompare.com.
Original VECTASTAIN® ABC Kit
Good sensitivity/low background
Original avidin/biotin ABC complex formulation
Low cost
Available without (“Standard” kit) or with biotinylated
species-specific secondary antibody.
Product review on Biocompare.com.
VECTASTAIN® Universal Quick Kits
The VECTASTAIN® Universal Quick Kit is designed for rapid
immunohistochemical staining of tissue sections. The kit relies
on a proprietary preformed peroxidase-streptavidin complex to
achieve outstanding sensitivity with short incubation times.
Rapid protocol: Staining in less than 20 minutes following
primary antibody incubation. Working solutions can be used
immediately after dilution.
High sensitivity, low background.
Biotinylated, Universal Pan-Specific secondary antibody
recognizes mouse, rabbit, and goat primary antibodies as
well as those from related species such as rat, bovine, and
sheep.*
Available in Ready-To-Use (R.T.U) format: Prediluted,
stabilized working solutions of VECTASTAIN® Universal Quick
Kit reagents provide the same high sensitivity and low
background as the concentrated VECTASTAIN® Universal
Kit.
* Do not use the pan-specific secondary antibody to stain rat, mouse or other
rodent, rabbit, goat, bovine, or sheep tissue due to potential reactivity with
endogenous IgG.
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