VECTASHIELD® Mounting Media are unsurpassed in preventing photobleaching. The different formulations of VECTASHIELD® Mounting Media all offer the same outstanding anti-fade and anti-photobleaching properties. They are all compatible with fluorescein, Texas Red®, AMCA, DyLight® dyes, Alexa Fluor® dyes, fluorescent nuclear stains, fluorescent proteins, fluorescent tracers, histochemical stains, and most fluorochromes.

Features of VECTASHIELD® Mounting Media:

  • Inhibit photobleaching of most fluorochromes
  • Available in non-hardening or hardening formulations
  • Available with or without DAPI or propidium iodide
  • No warming necessary
  • Slides can be viewed after prolonged storage
  • Continues to inhibit photobleaching after prolonged storage of mounted slides
  • Easy to use
  • Optically clear
  • Ideal refractive index

    The original VECTASHIELD® Mounting Medium is a glycerol-based, aqueous mountant that does not solidify but remains a viscous liquid on the slide. After mounting, coverslipped slides will not readily dry out and can be reviewed for weeks afterwards without sealing. For prolonged storage, coverslips can be permanently sealed around the perimeter with nail polish. Mounted slides should be stored at 4 ºC.

    VECTASHIELD® HardSet™ Mounting Medium is an aqueous mountant that hardens at room temperature in as little as 20 minutes. This facilitates handling of the slide, eliminates the need to secure the coverslip, and is more convenient for use with oil immersion microscopy. Stained and mounted slides may be stored for several weeks at 4 ºC; for prolonged storage, place slides at -20 ºC.

    Both the VECTASHIELD® HardSet™ and the original VECTASHIELD® Mounting Media are available with or without the counterstain DAPI (4’, 6-diamidino-2-phenylindole). DAPI produces a blue fluorescence when bound to DNA with excitation at about 360 nm and emission at 460 nm. The original VECTASHIELD® Mounting Medium is also available with the counterstain propidium iodide (PI). PI is a reddish nuclear counterstain with a broad excitation range at around 535 nm and emission at about 615 nm when bound to DNA.

    VECTASHIELD® HardSet™ and the original VECTASHIELD® Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins. Please consult our compatibility tables (located on specific product pages in pdf format) to determine if VECTASHIELD® will be compatible in your system.

    The optimal medium for super-resolution imaging methods maximizes the lifetime and photo-switching characteristics of fluorophores. VECTASHIELD Mounting Medium has been shown to be compatible with a number of fluorophores used in STORM imaging, such as Cy5 or Alexa fluor 647, resulting in greater convenience and reproducibility of the method. (Olivier, N. et al., 2013, Biomedical Optics Express, Vol. 4 No. 6, pp. 885-899).

     
  • Please note: VECTASHIELD® Mounting Medium may not be compatible with all enzymatic substrates or fluorescent proteins and test sections would be advisable when using anything other than commonly used fluorochromes.  
     
     
    VECTASHIELD® Mounting Medium Antifade Comparison

    Other manufacturers measure the antifade properties of their mountants using labeled microspheres or arrayed spots. Vector Labs prefers to measure antifade properties of VECTASHIELD® mountants using frozen tissue sections immunohistochemically stained with fluorescently labeled secondary antibodies.

    Antifade capability is measured using a 40x objective with real time imaging over 30 seconds of continuous exposure to the excitation illumination. Individual intensity measurements are recorded from 6 separate labeled regions and the average is calculated. The intensity after 30 second exposure is expressed as a percentage of the intensity at zero time. The values for PG are taken from the manufacturer’s published results.