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With over 35 years of research, development, and manufacturing experience Vector Laboratories has acquired considerable expertise in the production of immunofluorescence reagents. Our extensive range of fluorescent reagents accommodates a variety of experimental designs and levels of signal amplification.

Fluorochrome-Labeled Secondary Antibodies. Our affinity purified antibodies are unmatched in quality for immunological techniques. All antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. Antibodies are conjugated to ensure the optimal degree of labeling while not compromising the specificity or affinity of the antibody. See Figure 1.

Fluorochrome-Labeled Streptavidin and Avidin Systems. Fluorescent signals can be amplified using our biotinylated secondary antibodies followed by our highly purified fluorescent streptavidin and avidin conjugates. These fluorescent conjugates possess very low non-specific binding properties and extremely high affinity for biotin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody. See Figure 2.

Amplification of Fluorescent Signal using Biotinylated Anti-Streptavidin or Biotinylated Anti-Avidin. Biotinylated Anti-Streptavidin and Biotinylated Anti-Avidin antibodies provide an ideal method to further increase sensitivity. These antibodies bind to streptavidin or avidin, respectively, through both their antigen binding sites and also through the covalently attached biotin residues. After the first application of a fluorochrome-labeled streptavidin or avidin, the signal is amplified by incubation with Biotinylated Anti- Streptavidin or a Biotinylated Anti-Avidin antibody followed by a second incubation with fluorochrome-labeled streptavidin or avidin. This procedure results in the introduction of more fluorochromes at the target site. See Figure 3.  
 
Immunofluorescence continued