Vector Laboratories offers two lines of peroxidase substrates. Our original substrate kits provide sensitivity greater than conventional substrates and are known for their consistency and reliability. The reagents in the peroxidase substrate kits are supplied as concentrated stock solutions in convenient dropper bottles. The newest line, the ImmPACT™ substrates, provides the following advantages:

  • 2-4 fold greater sensitivity than original substrate kits
  • Supplied with stock solutions in dropper bottles and optimized diluent
  • Optimized specifically for immunohistochemistry applications
  • Working solution stable for up to 14 days at 4 ºC  
  •  
    Multiple Antigen Labeling

    Vector Laboratories is a leader in multiple antigen labeling applications because of our wide range of very sensitive and very low background detection reagents, our proprietary enzyme substrates, and our rigorously tested protocols. These detection systems and unique enzyme substrates offer an extensive choice of color combinations for multiple labeling using either:

  • The same enzyme system with different substrates to detect each antigen, or
  • Different enzyme systems and their substrates to detect each antigen.

    The following Enzyme Substrate Combinations Table is designed as a reference for optimal multiple labeling, because the order of the two colored precipitates can significantly affect the quality, color, and labeling pattern of each antigen in the stained section. This chart ensures that distinct colors are visible after the labeling reactions are completed using an optimized multiple labeling protocol.
     
  •