VECTASTAIN Elite ABC Video Tutorial
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Tissue staining using the VECTASTAIN Elite ABC system. For additional instructional videos please see the Tutorials page under the Support link. |
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About our VECTASTAIN ABC Systems:
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What is the "ABC Method"?
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Why is our ABC Method so sensitive?
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How do you use the "ABC Method"?
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What are some advantages of our ABC Method?
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What are some applications of the ABC System?
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What enzymes are used in the VECTASTAIN ABC Kits?
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How is a VECTASTAIN ABC Kit selected?
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What does each VECTASTAIN ABC Kit contain?
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What is a “Standard” VECTASTAIN ABC Kit?
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Which substrates should be used?
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Multiple Antigen Labeling in the Same Tissue Section
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Vector Laboratories has been at the forefront of developing novel, innovative labeling and detection reagents for over a quarter of a century. For immunohistochemistry, that accomplishment was first achieved with our VECTASTAIN® ABC kits and continues with the ImmPRESS™ polymerized reporter enzyme staining system. The wide popularity of these products is a consequence of their high sensitivity, low background, reliability, reproducibility and value pricing. Ease of use and versatility also enable the VECTASTAIN® ABC kits and ImmPRESS™ polymer detection reagents to be incorporated into applications such as multiple antigen labeling, without requiring significant procedural alterations or additional reagents.
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Accurate, reliable localization of two or more antigens on the same tissue section is a powerful research tool that can be easily applied in standard laboratory settings. The majority of multiple labeling applications use either enzyme or fluorescent-based techniques for visualization. This section, with an emphasis on the most sensitive detection methods, is intended as a guide to introduce double or triple labeling into the laboratory.
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A number of the reagents featured here are unique to Vector Laboratories. While other methods for multiple labeling not described here have been used in the past, the methods included herein provide optimal sensitivity coupled with exquisite color combinations, possible only with detection reagents from Vector Laboratories.
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Immunohistochemistry has certainly evolved over the last 25 years to become an invaluable technique in many facets of biological science. Through our expertise and diligence Vector Laboratories will continue to generate new products and methods to assist investigators in new discoveries. Please find detailed information about multiple antigen labeling, including protocols, in the link below.
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A Guide to Multiple Antigen Labeling (html browsing)
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A free printed brochure, "Discovery through color: A Guide to Multiple Antigen Labeling", is available for download below or upon request on our Catalog & Brochures request page under the Support menu. (Please note: this file is 8.5 MB).
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MLB.pdf (PDF)
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