The ImmPRESS™ polymerized reporter enzyme staining system
uses novel conjugation and micropolymer chemistries to create
a highly sensitive, ready-to-use, one-step, non-biotin detection
system. This unique micropolymer of highly active enzyme
is attached to our affinity purified secondary antibodies,
producing reagents with outstanding sensitivity and low
background.
The peroxidase micropolymers of the ImmPRESS™ reagent limit
steric interference and provide enhanced accessibility to the
target, avoiding the disadvantages of other polymer systems
that use large dextrans or other macromolecules as backbones.
The result is crisp, strong staining of antibody targets,
especially nuclear and membrane antigens (such as Ki67,
estrogen receptor, bcl-2, CD3, CD8 and CD10) and greater
sensitivity than other polymer systems.
The ImmPRESS™ Reagent is also available with alkaline phosphatase as the detection system. ImmPRESS™-AP (Alkaline Phosphatase) Polymer Reagents provide greater sensitivity than conventional alkaline phosphatase antibody conjugates and other alkaline phosphatase detection reagents for immunohistochemistry and other
applications.
Key advantages of the ImmPRESS™ Polymer System:
High sensitivity
Low background
Non-biotin
One-step detection
Ready-to-use in a convenient dropper bottle
Shorter assay time
Simplified multiple labeling
Blocking solution included
The ImmPRESS™ reagents are supplied prediluted and ready-to-
use in convenient dropper bottles along with prediluted
blocking serum. No mixing or titering of the ImmPRESS™
reagents is necessary to obtain optimal tissue section staining.
Consider Species Cross-Reactivity
When choosing the optimal detection system for your
application, it is important to consider not only the species of
the primary antibody but also the species of the tissue. If the
species of the primary antibody and the species of the tissue
are closely related (e.g. rat and mouse), the secondary antibody
may bind to endogenous IgG in the tissue section leading to
background. The following options minimize background
staining in these instances:
Use a secondary antibody specifically adsorbed to
remove cross-reacting antibodies of closely-related
species (e.g. ImmPRESS™ Anti-Mouse IgG, Rat Adsorbed).
Use the M.O.M.™ ImmPRESS™ Kit (Cat. No. MP-2400) for
applications of mouse primary antibodies on mouse
tissue.
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Staining Procedure
The staining procedure is simple as shown in the diagram below. Following a blocking step with the diluted normal horse serum, sections are incubated with primary antibody. After a brief wash, the appropriate ImmPRESS™ Reagent is added to the sections and incubated for 30 minutes. Sections are again rinsed and the slides are developed with the peroxidase substrate of choice.
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ImmPRESS Video Tutorial
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Tissue staining using the ImmPRESS system. For additional instructional videos please see the Tutorials page under the Support link. |
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Multiple Antigen Labeling Simplified
The ready-to-use, one-step, non-biotin, sensitive ImmPRESS™
Reagent significantly shortens staining times for multiple
antigen labeling. The reduced number of steps compared
to a conventional protocol directly decreases the amount of
slide handling. In systems containing endogenous biotin, the
ImmPRESS™ Reagent eliminates the need for avidin/biotin
blocking steps. Detailed information for multiple antigen
labeling can be found in our brochure, “ Discovery Through
Color: A Guide to Multiple Antigen Labeling” available for download or upon request on our Catalog & Brochures request page under the Support menu. (Please note: this file is 8.5 MB).
Product review on Biocompare.com.
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