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The ImmPRESS™ polymerized reporter enzyme staining system is based on a new method of polymerizing enzymes and attaching these polymers to antibodies. The novel approach employed to form enzyme "micropolymers" avoids the intrinsic shortcomings of using large dextrans or other macromolecules as backbones. Attaching a unique "micropolymer" with a high density of very active enzyme to a secondary antibody generates a reagent that overcomes steric interference and provides enhanced accessibility to its target. The result is outstanding sensitivity and signal intensity.
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Another feature of the ImmPRESS™ polymer detection reagents is low background staining. Elimination of a large backbone allows better diffusion of the reagents to the target sites and a reduction in non-specific binding. The ImmPRESS™ Anti-Mouse Ig, the ImmPRESS™ Anti-Rabbit Ig, and ImmPRESS™ Anti-Goat Ig reagents are composed of our ultra-pure affinity-purified secondary antibodies coupled with a unique "micropolymer" of a very active form of peroxidase. ImmPRESS™ Universal antibody reagent is a mixture of ImmPRESS™ anti-rabbit Ig and anti-mouse Ig reagents. ImmPRESS™ polymer detection reagents are biotin-free and avidin-free, reagents containing no biotin or avidin.
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The ImmPRESS™ reagents are supplied prediluted in a convenient dropper bottle format. No mixing or titering of the ImmPRESS reagents is necessary to obtain optimal tissue section staining. The ready-to-use reagents along with fewer steps in the staining procedure should save time without sacrificing intensity or compromising background quality.
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ImmPRESS™ Reagent kits consist of 50 ml of 2.5% normal horse blocking serum and 50 ml of the ready-to-use ImmPRESS™ Reagent.
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Staining Procedure:
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The staining procedure is simple as shown in the diagram below. Following a blocking step with the diluted normal horse serum, sections are incubated with primary antibody. After a brief wash, the appropriate ImmPRESS™ Reagent is added to the sections and incubated for 30 minutes. Sections are again rinsed and the slides are developed with the peroxidase substrate of choice.
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ImmPRESS Video Tutorial
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Tissue staining using the ImmPRESS system. For additional instructional videos please see the Tutorials page under the Support link. |
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Multiple Labeling:
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ImmPRESS™ Reagents can also be used for localizing two antigens in the same tissues sections, even using two antibodies of the same species. After one antigen has been reacted with the first primary antibody and localized with ImmPRESS™ Reagent and a peroxidase substrate, the tissue is blocked once again with diluted normal horse serum. The second primary antibody is now added, followed by incubation in the appropriate ImmPRESS™ Reagent. A different peroxidase substrate is then used to identify the location of the second antigen. By choosing peroxidase substrates that give the highest contrast and selecting the order of staining the two antigens, multiple labeling in the same tissue section using ImmPRESS™ reagents can be easily achieved.
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A free printed brochure, "Discovery through color: A Guide to Multiple Antigen Labeling", is available for download from the link below or upon request on our Catalog & Brochures request page under the Support menu. (Please note: this file is 8.5 MB).
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MLB.pdf (PDF)
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Product review on Biocompare.com.
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