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Featuring:
Plus
quality reagents for:
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Substrates (Alkaline Phosphatase) for Automated Staining |
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The alkaline phosphatase substrate kits which form precipitates are based on either reduction of tetrazolium salts or the production of
colored diazo compounds and provide approximately 200 ml of working solution. When stored at 4 ºC, the kits are stable for one year. These four substrate kits were developed to produce different colored precipitates which could all be permanently mounted in non-
aqueous media. Sections stained with the Vector® Red or Vector® Black substrate kits can be cleared with xylene. The Vector® Blue substrate product is soluble in xylene but can be cleared with citrus oil-based clearing agents and then permanently mounted. Three colors can be effectively introduced into a section to localize three antigens in different cells even using the same species of primary antibody, the same biotinylated secondary antibody and the
VECTASTAIN® ABC-AP reagent. The BCIP/NBT substrate is frequently used for nitrocellulose or in situ hybridization applications. Although these four substrates are excellent for most applications, they are not recommended for staining neuronal processes, fibers, or terminals.
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