DyLight® fluorescent dyes are direct alternatives to traditional fluorophores such as fluorescein (FITC) and rhodamine. In comparison, the DyLight® dyes offer a number of potential advantages including greater photostability and brighter fluorescence. Vector Labs have introduced these enhanced fluorescent compounds conjugated to affinity-purified secondary antibodies and streptavidin for use in a variety of applications in particular cell and tissue based immunofluorescent antigen detection/visualization.
The Dylight® dyes currently offered are DyLight® 488 (green), DyLight® 549 (orange), DyLight® 594 (red), and DyLight® 649 (far red). The excitation and emission spectra parallel that of other commercially available fluorescent reagents allowing for easy substitution into an existing protocol without requiring any further instrumentation or filter sets. Furthermore, the DyLight® dyes are completely stable over a pH range of 4-9 making them compatible with many aqueous-based buffers and diluents. The DyLight® dyes can be applied as single labels or in combination with other DyLight® dyes and fluorophores as part of a multiple immunofluorescent antigen staining methodology.
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