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| Quant*Tag Biotin Quantitation Kit
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The sample to be tested is reacted with the kit reagents along with standards containing known amounts of biotin. The absorbance readings of the known samples are plotted producing a standard curve. The absorbance of the test sample is located on the standard curve indicating the amount of biotin present.
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The Quant*Tag™ Biotin Kit (BDK-2000) is designed to
determine the amount of free biotin in solution or the
number of biotins attached to nucleic acids, proteins, or other
macromolecules. This kit can be used to determine accurately
the labeling efficiency of biotin-labeled molecules.
Unlike conventional biotin quantitation methods like the HABA
assay, no predigestion of protein or nucleic acids is required,
saving time and increasing accuracy. The Quant*Tag™ Biotin
Kit, more sensitive than the HABA assay, is able to detect less
than 1 nmol of biotin. Quant*Tag™ Kit reagents chemically
react with free or bound biotin producing a colored product
that can be quantified using a spectrophotometer. The
absorbance is measured in the visible spectrum allowing the
use of plastic cuvettes or microtitre plates.
The Quant*Tag™ Biotin Kit is quick and easy to use, and the
assay can be completed in 30 minutes. A biotin standard is
included. The kit contains sufficient reagents to perform from
25 to 250 tests depending on assay size.
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Biotin Quantitation using the QuantTag Biotin Kit
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Sensitive method of measuring biotin, free or bound to macromolecules using the QuantTag Kit. |
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Selected References:
Harris, EN, Weigel JA, Weigel PH. 2008. The human hyaluronan receptor for endocytosis (HARE/Stabilin-2) is a systemic clearance receptor for heparin. J Biol Chem. 283(25):17341-50.
Liu Y, Guan H, Beckett TL, Juliano MA, Juliano L, Song ES, Chow KM, Murphy MP, Hersh LB. 2007. In vitro and in vivo degradation of Aβ peptide by peptidases coupled to erythrocytes. Peptides. 28:2348-55.
Harris EN, Kyosseva SV, Weigel JA, Weigel PH. 2007. Expression, processing and glycosaminoglycan binding activity of the recombinant human 315-kDa hyaluronic acid receptor for endocytosis (HARE). J Biol Chem. 282(5):2785-97.
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