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Featuring:
Plus
quality reagents for:
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ImmPACT DAB Peroxidase Substrate
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Tonsil: Cytokeratin (m), ImmPRESS Anti-Mouse Ig Kit, ImmPACT DAB (brown) substrate. Hematoxylin (blue) counterstain.
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DAB (3, 3’-diaminobenzidine) produces a dark brown reaction
product and can be used for both immunohistochemical and
blotting applications. DAB is effective as a single label or as a
second color for multiple antigen labeling. Because of its heat-resistance, DAB can be used in IHC/
ISH double labeling applications. With the aid of imaging
systems and software, the spectral profile of DAB can be
distinguished from our other proprietary enzyme substrates
in applications where antigens are co-localized. Sections
stained with DAB can also be viewed by darkfield and electron
microscopy. Slides developed with DAB can be dehydrated,
cleared, and permanently mounted. The DAB reaction product
can be intensified with DAB Enhancing Solution (H-2200).
This product is an entirely new formula for a diaminobenzidine based
peroxidase substrate. ImmPACT™ DAB produces a
dark brown reaction product that is crisper and is 3-4 times
more sensitive than the original Vector® DAB substrate kit. It can be
used for both manual and automated staining methods. The
ImmPACT™ DAB working solution is stable for at least five
days at room temperature and at least two weeks when
stored at 4 °C. This product consists of 120 ml of diluent and a
convenient concentrated stock solution of highly purified DAB.
This product can also be used on blots.
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ImmPACT DAB Substrate Kit Video Tutorial
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Development of the ImmPACT DAB substrate from the ImmPACT DAB substrate kit. For additional instructional videos please see the Tutorials page under the Support link. |
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