DNA Molecular Weight Markers, Unlabeled

 
Price: $65.00
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Catalog # Unit Size Product Information MSDS
MB-1301    25 ug   Data Sheet   msdsMB1301.pdf  


This product is an electrophoresis standard consisting of 15 bands between 0.2 and 10 kilobase pairs. Bands are regularly spaced so that sizes are easy to identify. Markers are supplied ready-to-use containing loading dye so that electrophoresis can be monitored.

The Unlabeled DNA Molecular Weight Marker (MB-1301) is used when the marker is to be visualized in a gel by ethidium bromide or other DNA stains.

Ready-to-use marker (5 μl or 500 ng) was loaded on a 0.5% agarose gel. The gel was run in 1x TBE at 10 V/ cm, stained with ethidium bromide (left) or blotted onto nylon membrane using standard methods. Biotinylated DNA bands (right) were detected using the UltraSNAP™ Kit (Alkaline Phosphatase Streptavidin followed by DuoLuX™ Chemiluminescent Substrate).