Vector Laboratories’ fluorochrome-conjugated streptavidin and
avidin reagents are highly purified and possess very low non-specific
binding properties. They have extremely high affinity
for biotin. These fluorescent conjugates can be used to detect
biotinylated secondary antibodies and other macromolecules in
applications such as immunofluorescence, in situ hybridization,
or flow cytometry.
Amplification of fluorescent signals can be easily achieved with
our biotinylated secondary antibodies followed by our highly
purified fluorochrome-labeled streptavidin or avidin. Using a
biotin/avidin or biotin/streptavidin detection system results in
an additional layer of amplification over a directly conjugated
DyLight® dyes offer several advantages including greater
photostability, pH independence, and brighter fluorescence.
We offer DyLight® conjugated streptavidin for use in a
variety of applications, in particular, cell- and tissue-based
immunofluorescent detection. The DyLight® conjugates are
completely stable from pH 4 to pH 9, making them compatible
with many aqueous-based buffers and diluents.