Vector Labs: Agarose bound* Dolichos Biflorus Agglutinin (DBA)

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	*Agarose bound Dolichos Biflorus Agglutinin (DBA)**

Price:	$125.00
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Colon: Dolichos biflorus agglutinin (black, DAB+Ni), Alcian blue counterstain.

Catalog #	Unit Size	Product Information	MSDS
AL-1033	2 ml	Data Sheet Lectin Properties Glycoprotein Eluting Solutions Compatibility Chart	msdsAL1033.pdf

Dolichos biflorus agglutinin is a glycoprotein with a molecular weight of about 120,000 and consists of 4 subunits of approximately equal size. This lectin has an isoelectric point of about pH 5.5 and a carbohydrate specificity toward α-linked N-acetylgalactosamine. DBA is approximately 5000 times more active in agglutinating blood group A1 cells versus A2 cells, and is used to distinguish these blood types in routine serology. It has also been used to establish secretor status in A1 individuals by hemagglutination inhibition techniques and has potential applications in examining tumors which affect the levels of blood group substances.

Agarose bound* Dolichos biflorus agglutinin is prepared using our affinity-purified lectins. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2x10⁷ daltons are used as the solid-phase matrix to which the lectins are covalently coupled. The attachment of the lectins to the beads is carefully controlled to preserve lectin activity and minimize conformational changes of the bound lectins that might result in nonspecific ionic or hydrophobic interactions. The technique we have developed to couple lectins to agarose beads inserts a hydrophilic spacer arm between the lectin and the matrix.

This coupling method provides several advantages over the traditional cyanogen bromide procedure:

Maximum carbohydrate binding activity of the coupled lectins is retained

Linkage is stable over a range of pH values

Conjugated proteins are not leached off the beads by Tris or other routinely used buffers

No residual charges are present after conjugation. This minimizes non-specific binding to the matrix.

Our agarose bound lectins are supplied at a constant concentration of lectin per ml of settled beads. The concentration for each lectin is selected to achieve the highest glycoconjugate binding capacity per mg of lectin present in the beads. Each lot is tested for its binding capacity using glycoproteins known to bind the lectin. This provides a guideline for the user and assures the quality of our agarose bound lectins.

Inhibiting/Eluting Sugar: 200 mM N-acetylgalactosamine

*3 mg lectin/ml gel