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Featuring:
Plus
quality reagents for:
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| Agarose bound* Erythrina Cristagalli Lectin (ECL, ECA)
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Erythrina cristagalli lectin consists of two different subunits
of approximately 28 kDa and 26 kDa. The carbohydrate
structure to which ECL binds is frequently
found in membrane
and serum glycoproteins of mammalian origin. Sialic acid
substitution on this structure appears to prevent the lectin from
binding. This specificity offers an opportunity to utilize agarose
bound ECL to isolate or fractionate mammalian glycoproteins.
This lectin has been reported to be useful for the isolation
of human natural killer (NK) cells using a negative selection
panning technique (protocol available upon request or on our
website). Human NK cells appear to lack accessible surface
carbohydrate structures required for binding ECL and, unlike
other mononuclear cells, do not adhere to ECL-coated culture
dishes. Since this procedure involves a negative selection
panning technique, a high recovery of viable NK cells can
be obtained. The adherent cells can also be recovered by
incubation in galactose or lactose.
Agarose bound* Erythrina cristagalli lectin is prepared using our affinity-purified
lectins. Heat stable, cross-linked 4% agarose beads with
a molecular weight exclusion limit of about 2x107 daltons
are used as the solid-phase matrix to which the lectins are
covalently coupled. The attachment of the lectins to the beads
is carefully controlled to preserve lectin activity and minimize
conformational changes of the bound lectins that might result
in nonspecific ionic or hydrophobic interactions. The technique
we have developed to couple lectins to agarose beads inserts a
hydrophilic spacer arm between the lectin and the matrix.
This coupling method provides several advantages over the
traditional cyanogen bromide procedure:
Maximum carbohydrate binding activity of the coupled
lectins is retained
Linkage is stable over a range of pH values
Conjugated proteins are not leached off the beads by Tris or
other routinely used buffers
No residual charges are present after conjugation. This
minimizes non-specific binding to the matrix.
Our agarose bound lectins are supplied at a constant
concentration of lectin per ml of settled beads. The
concentration for each lectin is selected to achieve the highest
glycoconjugate binding capacity per mg of lectin present in
the beads. Each lot is tested for its binding capacity using
glycoproteins known to bind the lectin. This provides a
guideline for the user and assures the quality of our agarose
bound lectins.
Inhibiting/Eluting Sugar: 200 mM lactose
*3 mg lectin/ml gel
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