Agarose bound* Griffonia (Bandeiraea) Simplicifolia Lectin I (GSL I, BSL I)

 
Price: $135.00
Quantity:
 
 


Catalog # Unit Size Product Information MSDS
AL-1103    2 ml   Data Sheet
Lectin Properties
Glycoprotein Eluting Solutions Compatibility Chart  
AL-1103eumsds.pdf  


GSL I is a family of glycoproteins with molecular weights of approximately 114 kDa. There are two types of subunits, termed “A” and “B”, with slightly different molecular weights. These subunits combine to form tetrameric structures, resulting in five isolectins. The “A”-rich lectin preferentially agglutinates blood group A erythrocytes and thus appears to be specific for α-N-acetylgalactosamine residues, while the “B”-rich lectin preferentially agglutinates blood group B cells and is specific for α-galactose residues. Our GSL I is a mixture of the five isolectins. GSL I has been reported to bind several glycoproteins including laminin.

Agarose bound* GSL I is prepared using our affinity-purified lectins. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2x107 daltons are used as the solid-phase matrix to which the lectins are covalently coupled. The attachment of the lectins to the beads is carefully controlled to preserve lectin activity and minimize conformational changes of the bound lectins that might result in nonspecific ionic or hydrophobic interactions. The technique we have developed to couple lectins to agarose beads inserts a hydrophilic spacer arm between the lectin and the matrix.

This coupling method provides several advantages over the traditional cyanogen bromide procedure:

  • Maximum carbohydrate binding activity of the coupled lectins is retained
  • Linkage is stable over a range of pH values
  • Conjugated proteins are not leached off the beads by Tris or other routinely used buffers
  • No residual charges are present after conjugation. This minimizes non-specific binding to the matrix.

    Our agarose bound lectins are supplied at a constant concentration of lectin per ml of settled beads. The concentration for each lectin is selected to achieve the highest glycoconjugate binding capacity per mg of lectin present in the beads. Each lot is tested for its binding capacity using glycoproteins known to bind the lectin. This provides a guideline for the user and assures the quality of our agarose bound lectins.

    Inhibiting/Eluting Sugar: mixture of 200 mM galactose/200 mM N-acetylgalactosamine

    *4 mg lectin/ml gel

  • Related Products:
    Biotinylated Griffonia (Bandeiraea) Simplicifolia Lectin I (GSL I, BSL I)
    BSA-Galactose
    Fluorescein labeled Griffonia (Bandeiraea) Simplicifolia Lectin I (GSL I, BSL I)
    galactose
    Glycoprotein Eluting Solution for Galactose- or GalNAc-binding Lectins
    N-acetylgalactosamine
    Rhodamine labeled Griffonia (Bandeiraea) Simplicifolia Lectin I (GSL I, BSL I)
    Unconjugated Griffonia (Bandeiraea) Simplicifolia Lectin I(GSL I, BSL I)