Agarose bound* Jacalin

 
Size Price Qty  
10ml $320.00
2ml $115.00
5ml $195.00
 

Small intestine: Jacalin (brown, DAB), Vector Hematoxylin counterstain.

Catalog # Unit Size Product Information MSDS
AL-1153  10ml
2ml
5ml
 
Data Sheet (5 ml)
Data Sheet (10 ml)
Data Sheet (2ml)
Lectin Properties
Glycoprotein Eluting Solutions Compatibility Chart
Specificity of Jacalin binding  
msdsAL1153.pdf  


Jacalin is a lectin composed of four subunits of approximately 16 kDa each. This lectin appears to bind only O-glycosidically linked oligosaccharides, preferring the structure galactosyl (β-1,3) N-acetylgalactosamine. This structure (the T-antigen) is the oligosaccharide to which peanut agglutinin (PNA) binds. However, unlike PNA, Jacalin will bind a mono- or disialylated form of this structure. This lectin has been used to purify human IgA. The specificity of this lectin also affords the opportunity to localize or isolate glycoproteins with O-glycosidically linked oligosaccharide side chains.

Agarose bound* Jacalin is prepared using our affinity-purified lectins. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2x107 daltons are used as the solid-phase matrix to which the lectins are covalently coupled. The attachment of the lectins to the beads is carefully controlled to preserve lectin activity and minimize conformational changes of the bound lectins that might result in nonspecific ionic or hydrophobic interactions. The technique we have developed to couple lectins to agarose beads inserts a hydrophilic spacer arm between the lectin and the matrix.

This coupling method provides several advantages over the traditional cyanogen bromide procedure:

  • Maximum carbohydrate binding activity of the coupled lectins is retained
  • Linkage is stable over a range of pH values
  • Conjugated proteins are not leached off the beads by Tris or other routinely used buffers
  • No residual charges are present after conjugation. This minimizes non-specific binding to the matrix.

    Our agarose bound lectins are supplied at a constant concentration of lectin per ml of settled beads. The concentration for each lectin is selected to achieve the highest glycoconjugate binding capacity per mg of lectin present in the beads. Each lot is tested for its binding capacity using glycoproteins known to bind the lectin. This provides a guideline for the user and assures the quality of our agarose bound lectins.

    Inhibiting/Eluting Sugar: 800 mM galactose or 100 mM melibiose

    *4 mg lectin/ml gel

  • Related Products:
    Biotinylated Jacalin
    BSA-Galactose
    Fluorescein labeled Jacalin
    galactose
    Glycoprotein Eluting Solution for Galactose- or GalNAc-binding Lectins
    Unconjugated Jacalin