| |
Featuring:
Plus
quality reagents for:
|
|
| |
 |
 |
| Agarose bound* Phaseolus vulgaris Erythroagglutinin (PHA-E)
|
|
|
|
Phaseolus vulgaris agglutinin is the name ascribed to a family
of lectins, each of which consists of four subunits. There are
two different types of subunits. One appears to be involved
primarily in red cell agglutination and has been designated the
“E” subunit (for erythroagglutinin). The other type is involved
in lymphocyte agglutination and mitogenic activity and has
been termed the “L” subunit (for leucoagglutinin).
These subunits combine to produce five isolectins.
One of these isolectins has four “E” subunits and is designated
PHA-E. PHA-E possesses strong hemagglutinating activity
but is a poor mitogen. PHA-L, with four “L” type subunits,
does not agglutinate red cells but is a potent mitogen. The
other three isolectins, designated E3L1, E2L2, and E1L3, have
erythroagglutinating and mitogenic activities proportional to
the number of respective “E” or “L” subunits. We have termed
the mixture of the five isolectins PHA (E+L).
PHA-L has been found to be an excellent, specific marker for
use in anterograde neuronal tracing.
Agarose bound* PHA-E is prepared using our affinity-purified
lectins. Heat stable, cross-linked 4% agarose beads with
a molecular weight exclusion limit of about 2x107 daltons
are used as the solid-phase matrix to which the lectins are
covalently coupled. The attachment of the lectins to the beads
is carefully controlled to preserve lectin activity and minimize
conformational changes of the bound lectins that might result
in nonspecific ionic or hydrophobic interactions. The technique
we have developed to couple lectins to agarose beads inserts a
hydrophilic spacer arm between the lectin and the matrix.
This coupling method provides several advantages over the
traditional cyanogen bromide procedure:
Maximum carbohydrate binding activity of the coupled
lectins is retained
Linkage is stable over a range of pH values
Conjugated proteins are not leached off the beads by Tris or
other routinely used buffers
No residual charges are present after conjugation. This
minimizes non-specific binding to the matrix.
Our agarose bound lectins are supplied at a constant
concentration of lectin per ml of settled beads. The
concentration for each lectin is selected to achieve the highest
glycoconjugate binding capacity per mg of lectin present in
the beads. Each lot is tested for its binding capacity using
glycoproteins known to bind the lectin. This provides a
guideline for the user and assures the quality of our agarose
bound lectins.
Elution: 100 mM acetic acid
*3 mg lectin/ml gel
|
|
|
|
|
Related
Products:
|
|
|
|