Agarose bound*, succinylated Wheat Germ Agglutinin (WGA)

 
Size Price Qty  
2ml $130.00
5ml $230.00
 


Catalog # Unit Size Product Information MSDS
AL-1023S  2ml
5ml
 
Lectin Properties
Glycoprotein Eluting Solutions Compatibility Chart
Data Sheet (2 ml)
Data Sheet (5 ml)  
AL-1023Seumsds.pdf  


This derivative has been reported to have properties distinct from the native lectin. Evidence suggests that Succinylated Wheat Germ agglutinin does not bind to sialic acid residues, unlike the native form, but retains its specificity toward N-acetylglucosamine. Using conjugates of the native lectin and the succinylated form can provide a system to distinguish between sialylated glycoconjugates and those containing only N-acetylglucosamine structures.

Agarose bound*, succinylated WGA is prepared using our affinity-purified lectins. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2x107 daltons are used as the solid-phase matrix to which the lectins are covalently coupled. The attachment of the lectins to the beads is carefully controlled to preserve lectin activity and minimize conformational changes of the bound lectins that might result in nonspecific ionic or hydrophobic interactions. The technique we have developed to couple lectins to agarose beads inserts a hydrophilic spacer arm between the lectin and the matrix.

This coupling method provides several advantages over the traditional cyanogen bromide procedure:

  • Maximum carbohydrate binding activity of the coupled lectins is retained
  • Linkage is stable over a range of pH values
  • Conjugated proteins are not leached off the beads by Tris or other routinely used buffers
  • No residual charges are present after conjugation. This minimizes non-specific binding to the matrix.

    Our agarose bound lectins are supplied at a constant concentration of lectin per ml of settled beads. The concentration for each lectin is selected to achieve the highest glycoconjugate binding capacity per mg of lectin present in the beads. Each lot is tested for its binding capacity using glycoproteins known to bind the lectin. This provides a guideline for the user and assures the quality of our agarose bound lectins.

    Inhibiting/Eluting Sugar: Chitin Hydrolysate (Cat. No. SP-0090) or 500 mM N-acetylglucosamine with salt and/or acid elution generally required

    *3 mg lectin/ml gel

  • Related Products:
    Biotinylated, succinylated Wheat Germ Agglutinin (WGA)
    BSA-GlcNAc (BSA-N-Acetylglucosamine)
    Chitin Hydrolysate
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    Glycoprotein Eluting Solution for GlcNAc or Chitin-binding Lectins
    N-acetylglucosamine
    Rhodamine labeled, succinylated Wheat Germ Agglutinin (WGA)
    Succinylated Wheat Germ Agglutinin (WGA)