VECTASTAIN Elite ABC Kit (Rabbit IgG)

 
Price: $345.00
Quantity:
 
 


Small Bowel: Ki67 antigen stained using VECTASTAIN Elite ABC Kit and Vector DAB (brown) substrate. Hematoxylin QS (blue) counterstain.

Catalog # Unit Size Product Information MSDS
PK-6101    1 kit   Multiple Antigen Labeling Guide
Protocol
Enzyme Immunoassays, Hybridoma Screening, and Western Blots
Troubleshooting Guide
Enzyme Substrate Combinations Chart
Quenching Endogenous Peroxidase Activity  
PK-6100series.eumsds.pdf  


VECTASTAIN Elite ABC Video Tutorial

  Tissue staining using the VECTASTAIN Elite ABC system. For additional instructional videos please see the Tutorials page under the Support link.
With more than 40,000 citations to its credit, the VECTASTAIN® ABC kit remains widely popular. Based on the versatile biotin/avidin interaction, the system is modular, and along with our selection of secondary antibodies, can accommodate a wide array of primary antibody and tissue species. Our ABC kits are economical and continue to be a staple product in any immunohistochemistry laboratory.

Peroxidase substrates produce sharp, dense precipitates with crisp localization. These characteristics, in conjuction with the high sensitivity and low background of the VECTASTAIN® ABC systems, make the peroxidase enzyme a preferred choice in many applications. (eg. In neural tissue, the peroxidase system is often preferred because it gives more consistent labeling of both cell bodies and processes.)

VECTASTAIN® Elite® ABC System

  • Advanced avidin/biotin technology: The Elite® ABC complex is smaller, very uniform, and highly active, allowing more accessibility for binding to a biotinylated target.

  • Highest sensitivity, low background: The VECTASTAIN® Elite® ABC system is the most sensitive avidin/biotin-based peroxidase system. The Elite® ABC series is approximately 5 times more sensitive than the original VECTASTAIN® ABC Kit with the same low background.

  • Cost effective: Higher sensitivity leads to lower cost per slide.

  • Available without (“Standard” kit) or with biotinylated species specific or universal secondary antibodies.

  • Available in Ready-To-Use (R.T.U.) formats: Prediluted, stabilized working solutions of Elite® ABC Kit reagents provide the same high sensitivity and low background as the traditional VECTASTAIN® Elite® ABC Kit reagents.
  • All of the VECTASTAIN® Elite ABC kits can be used on frozen or paraffin-embedded tissues, cell smears, or cytospin preparations.
    Kit Contents:

  • 3 ml normal goat serum
  • 1 ml of concentrated biotinylated goat anti-rabbit IgG secondary antibody
  • 2 ml each of concentrated A and B reagents.

    Sufficient reagents to stain approximately 500-1000 sections.
  • Product review on Biocompare.com.

    For additional information on the VECTASTAIN® ABC systems, please click on the link below.
    VECTASTAIN ABC Systems

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