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| VECTOR Blue Alkaline Phosphatase Substrate Kit
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Breast Carcinoma (triple label): Estrogen Receptor (m), VECTASTAIN Elite ABC Kit, Vector NovaRED substrate (red); CD34 (m), VECTASTAIN ABC-AP Kit, Vector Blue substrate (blue); Cytokeratin 8/18 (m), VECTASTAIN Elite ABC Kit, DAB substrate (brown).
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The Vector® Blue Substrate Kit produces a blue, heat
stable reaction product. This substrate can be used singly or
in combination with other alkaline phosphatase or peroxidase
substrates for multiple label applications. With the aid of imaging systems and software, the
spectral profile of this substrate can be distinguished from
other enzyme substrates in applications where antigens
are co-localized. Vector® Blue is also fluorescent. Sections
stained with the Vector® Blue substrate can be dehydrated,
cleared, and permanently mounted; however, non-xylene
clearing agents and non-xylene based mounting media (such
as VectaMount™, H-5000) must be used because the reaction
product is soluble in xylene. Vector® Blue can also be used on
blots in applications such as ELISPOT, singly or in combination
with a peroxidase substrate such as AEC. This kit contains
stock solutions in convenient dropper bottles.
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