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Featuring:
Plus
quality reagents for:
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| VECTREX AAL Reversible Nucleic Acid Binding Matrix
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For Fucose Labeled Nucleic Acids
VECTREX® AAL (MB-1396) is used for reversible binding
of fucose-labeled nucleic acids. Binding and elution can
be performed at physiological pH and salt concentrations.
VECTREX® AAL contains Aleuria aurantia lectin and will bind
nucleic acids labeled with fucose using either the FastTag®
system, or 3’ or 5’ EndTag™ Labeling Systems.
Elution is easily accomplished under non-denaturing conditions
with L-fucose. The matrix is supplied as a 1:1 suspension in
buffer. The binding capacity is at least 25 ng fucose-labeled λ
DNA per μl 1:1 suspension.
The VECTREX® matrix is optimized for immobilization of nucleic
acids. This matrix consists of particles of a highly cross-linked
sugar polymer that has a very large surface area and low nonspecific
binding for nucleic acids. Unlike agarose, which may
retain small molecules in its pores, the VECTREX® particle will
retain molecules only through specific affinity interaction on
the surface of the particles. The VECTREX® matrix is dense and
sediments readily even without centrifugation.
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The Aleuria aurantia lectin conjugated to VECTREX® particles
reversibly binds fucose. Thus fucosylated nucleic acids or other molecules can be bound to VECTREX® AAL under a wide variety of conditions and subsequently eluted with fucose under non-denaturing conditions. Potential applications include probe purification, PCR, cDNA and subtraction library construction, hybridization analysis, and in vitro mutagenesis.
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Reversible binding of FastTag™ Fucose-labeled l Hind III DNA to VECTREX® AAL. Labeled (+) or unlabeled (-) DNA was incubated with VECTREX® AAL (+) or binding buffer (-). Following centrifugation of binding reactions, VECTREX® AAL was washed and the bound DNA eluted with L-fucose. Supernatants and eluates from the binding reactions were fractionated by agarose gel electrophoresis and DNA visualized by ethidium bromide staining.
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