VECTREX AAL Reversible Nucleic Acid Binding Matrix

 
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Catalog # Unit Size Product Information MSDS
MB-1396    1 ml   Protocol
Data Sheet
Data Sheet 2  
MB-1396eumsds.pdf  


For Fucose Labeled Nucleic Acids

VECTREX® AAL (MB-1396) is used for reversible binding of fucose-labeled nucleic acids. Binding and elution can be performed at physiological pH and salt concentrations. VECTREX® AAL contains Aleuria aurantia lectin and will bind nucleic acids labeled with fucose using either the FastTag® system, or 3’ or 5’ EndTag™ Labeling Systems. Elution is easily accomplished under non-denaturing conditions with L-fucose. The matrix is supplied as a 1:1 suspension in buffer. The binding capacity is at least 25 ng fucose-labeled λ DNA per μl 1:1 suspension.

The VECTREX® matrix is optimized for immobilization of nucleic acids. This matrix consists of particles of a highly cross-linked sugar polymer that has a very large surface area and low nonspecific binding for nucleic acids. Unlike agarose, which may retain small molecules in its pores, the VECTREX® particle will retain molecules only through specific affinity interaction on the surface of the particles. The VECTREX® matrix is dense and sediments readily even without centrifugation.    
The Aleuria aurantia lectin conjugated to VECTREX® particles reversibly binds fucose. Thus fucosylated nucleic acids or other molecules can be bound to VECTREX® AAL under a wide variety of conditions and subsequently eluted with fucose under non-denaturing conditions. Potential applications include probe purification, PCR, cDNA and subtraction library construction, hybridization analysis, and in vitro mutagenesis.
Reversible binding of FastTag™ Fucose-labeled l Hind III DNA to VECTREX® AAL. Labeled (+) or unlabeled (-) DNA was incubated with VECTREX® AAL (+) or binding buffer (-). Following centrifugation of binding reactions, VECTREX® AAL was washed and the bound DNA eluted with L-fucose. Supernatants and eluates from the binding reactions were fractionated by agarose gel electrophoresis and DNA visualized by ethidium bromide staining.