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Featuring:
Plus
quality reagents for:
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| Biotinylated Anti-Streptavidin
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Our antibodies to avidin and streptavidin are produced in goats
using our highly purified avidin or streptavidin and isolated by
affinity chromatography. Anti-Avidin does not bind streptavidin
and Anti-Streptavidin does not recognize avidin. These
antibodies provide opportunities to significantly amplify signals
in many applications.
Biotinylated Anti-Avidin and Biotinylated Anti-Streptavidin
have been widely used as amplifying reagents in
immunohistochemistry, in situ hybridization, microarray assays,
ELISAs, blots, and many other applications. The capability of
binding avidin or streptavidin via either biotin binding sites
or through antigen binding sites, makes these biotinylated
antibodies unique. These antibodies can be used either
as part of preformed complexes or in sequence to amplify
fluorescent signals. When used in sequence, the target is first
labeled with fluorochrome-conjugated avidin or streptavidin,
followed by incubation with Biotinylated Anti-Avidin or
Biotinylated Anti-Streptavidin, followed by a second layer of
fluorochrome-conjugated avidin or streptavidin. This sequence
can be repeated. This multi-layered approach introduces more
fluorochromes at the target site and can provide a multi-fold
amplification over a single layer.
These affinity purified antibodies are also available
unconjugated or fluorescein-labeled.
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Step 1: Add Fluorescent Streptavidin
Step 2: Add Biotinylated Anti-Streptavidin
Step 3: Add Fluorescent Streptavidin
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