/ ImmunofluorescenceA Guide to Multiple Antigen Labeling (html browsing)
Vector Laboratories offers many solutions for the localization of two or more antigens in the same tissue section. Choosing the optimal method will depend on several factors:
the type of tissue being stained
whether the antigens are co-localized in the same cellular compartment
the limitations in sensitivity of a staining method
In general, immunoenzymatic methods can be used to stain two or more antigens in the same tissue section when the antigens are located in different cell types or different compartments of the same cell. In cases where the two antigens are co-localized in the same compartment of the same cell, e.g., two nuclear antigens, immunofluorescence techniques may be preferred. Please click on the links below for information and protocols.