Fusion protein reagents are part of a methodology for
purification, detection, and investigation of expressed proteins.
The common fusion tags [green fluorescent protein (GFP), poly
histidine (polyHis), c-Myc, human influenza virus hemagglutinin
(HA), Maltose binding protein (MBP)] can be easily detected
with our antibodies against the specific tag.
Our biotinylated, affinity-purified polyclonal antibodies
(produced in goat) are optimized to detect fusion proteins
in western blot detection. They can be used in combination
with a VECTASTAIN® ABC-AmP™ detection system or other
For maximum sensitivity, GFP can also be detected using
ImmPRESS™ Anti-GFP antibody (made in goat). This reagent
is prepared using our proprietary peroxidase micropolymer
Recombinant GFP Standard (MB-0752) contains purified
recombinant Aquorea victorea GFP (28 kDa) overexpressed
in E. coli. It is designed for quantitation of GFP fusion protein
in the test sample, and can be used as posistive control on
western blots in conjuction with Peroxidase-labeled Anti-GFP
(MB-0712) or Biotinylated Anti-GFP (BA-0702).
Fusion Protein Purification. The Fusion-Aid™ Kits are
designed to immunoprecipitate/isolate fusion proteins
containing the appropriate protein tag. The Fusion-Aid™
Kit has affinity-purified primary antibodies coupled directly
to agarose beads. In contrast to traditional methods, this
eliminates the contamination of the isolated proteins with
coprecipitating primary antibodies. The spin columns provided
with the kit also facilitate rapid immunoprecipitation and
maximum protein recovery.