Since 1976, Vector Laboratories has been at the forefront
of developing innovative detection reagents. The
VECTASTAIN® ABC systems established us as the leader in
immunohistochemistry. Because of our commitment to
developing novel technologies and products of consistent
quality, Vector Laboratories remains a trusted name in this
field. Our products are well regarded for their high sensitivity,
low background, reliability, reproducibility, and value pricing.
Many different detection systems are available from us to
accommodate the wide range of experimental priorities.
VECTASTAIN® ABC Systems. With more than 40,000
citations to its credit, the VECTASTAIN® ABC kit remains widely
popular. Based on the versatile biotin/avidin interaction, the
system is modular, and along with our selection of secondary
antibodies, can accommodate a wide array of primary antibody
and tissue species. Our ABC kits are economical and continue
to be a staple product in any immunohistochemistry laboratory.
ImmPRESS™ Peroxidase Polymer Reagents. The highly sensitive,
one-step, ready-to-use, non-biotin, ImmPRESS™ Polymer
Detection reagents are the result of novel conjugation methods
and micropolymer chemistry developed at Vector Laboratories.
Peroxidase micropolymers allow excellent resolution and crisp,
strong staining of antigens, especially nuclear and membrane
antigens. ImmPRESS™ reagents avoid the steric problems of
other polymer-based reagents, thus providing superior results.
These reagents are ideal for multiple antigen labeling because
of the simplified one-step protocol.
Vector® M.O.M.™ (Mouse on Mouse) Immunodetection
System. The specific detection of mouse primary antibodies
on mouse tissue is difficult due to the presence of endogenous
mouse immunoglobulins. A conventional anti-mouse detection
system cannot distinguish between the mouse primary
antibody and the endogenous mouse immunoglobulins
leading to high background. The Vector® M.O.M.™ system is
designed for the localization of mouse primary antibodies on
mouse tissue. This system uses a VECTASTAIN®ABC staining
method but contains a proprietary Mouse Ig Blocking Reagent
and a specially modified anti-mouse Ig secondary antibody.
Tedious calculations and primary antibody pre-binding steps
are not required. Excellent staining results for a once difficult
application have now become routine with the Vector®
Proprietary Enzyme Substrates. Along with the traditional
chromogenic substrates Vector Laboratories also offers a wide
variety of proprietary enzyme substrates. These proprietary
substrates provide novel color choices essential for staining
antigens in pigmented tissues and melanomas as well as for
localizing multiple antigens in the same tissue section. Several
substrates can be effectively viewed under darkfield or electron
microscopy. Many of our substrates have unique spectral
profiles that may be useful in the co-localization of antigens
using spectral imaging systems.
Multiple Antigen Labeling. Accurate, reliable localization of
two or more antigens on the same tissue section is a powerful
research tool that can be applied in standard laboratory
settings. The high specificity and low background of our
reagents and proprietary enzyme substrates provide optimal
sensitivities coupled with exquisite color combinations. Many
of our substrates allow co-localization of antigens using