NEUROBIOTIN™ Tracer (SP-1120) is an amino derivative
of biotin that can be used as an intracellular label for
cells, particularly neurons. It is used for visualizing neural
architecture and for the identification of gap junction coupling.
Key advantages of NEUROBIOTIN™ Tracer over biocytin and
other neuronal labels:
Better solubility
More efficiently iontophoresed
Remains in cell longer
Non-toxic
Can be fixed with formalin or glutaraldehyde
NEUROBIOTIN™ Tracer can be used in many types of
preparations including in vivo, whole mounts, slice
preparations, or cultured cells. It can be delivered by many
routes such as intracellular electrodes, microinjection, cutloading,
or scrape-loading. NEUROBIOTIN™ Tracer can be
detected using avidin or streptavidin systems with either
chromogenic or fluorescence visualization methods.
|