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VECTASTAIN ABC-AmP Kit, for Mouse IgG, Chromogenic Western Blot Detection

 
Price: £191.00
Quantity:
  		 

 

Catalog # Unit Size Product Information MSDS
AK-6402    1 kit   Western Blot Troubleshooting Guide
Protocol (CE)   		AK-6000series.eumsds.pdf  


Greater sensitivity can be achieved by using an amplified detection procedure. Our biotin/avidin and biotin/streptavidin systems introduce a large number of enzymes to the target site providing signal amplification while maintaining low background.

The VECTASTAIN® ABC-AmP™ is an amplified ABC alkaline phosphatase reagent for the detection of mouse or rabbit primary antibodies on nitrocellulose or PVDF membranes. The signal can be visualized using either a chemiluminescent/ chemifluorescent or a chromogenic substrate. Used with the DuoLuX™ Chemiluminescent/Chemifluorescent Substrate, the VECTASTAIN® ABC- AmP™ system produces a very high and sustained light emission signal, with low background, and permanent fluorescence.

The sensitivity of VECTASTAIN® ABC-AmP™ Reagent combined with the DuoLux™ substrate allows for the detection of as little as 1 pg of target protein.

Each VECTASTAIN® ABC-AmP™ kit provides sufficient reagents to develop approximately twenty 100 cm2 blots. Kit consists of

  • VECTASTAIN® ABC-AmP™ reagent
  • 10x casein solution
  • biotinylated anti-mouse IgG secondary antibody
  • BCIP/NBT chromogenic substrate

    Reagents contained in the kits can also be purchased separately.      
    •  
    Western blot visualized by using the VECTASTAIN® ABC-AmP™ kits. Serial dilutions (1:2) of maltose binding protein (MBP) were resolved by electrophoresis on a 12% PAGE reducing gel, transferred onto nitrocellulose membrane and detected with biotinylated anti-MBP made in goat, followed by the VECTASTAIN® ABC-AmP™ Reagent. Blots were developed with [A] DuoLuX™ Substrate using chemiluminescence, or [B] BCIP/NBT substrate.