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Featuring:
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quality reagents for:
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| PHOTOPROBE Biotin Nucleic Acid Labeling and Detection System
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PHOTOPROBE Biotin labeling compared to 32P labeling in a Southern analysis. 5 µg, 0.5 µg, 0.05 µg of E. coli genomic DNA was restriction digested, electrophoresed, visualized with EtBr (A), and transferred to nylon membrane. (B) The E.coli FabG gene was detected with a probe labeled with PHOTOPROBE Biotin followed by Alkaline Phosphatase Streptavidin. Chemiluminescence was detected with a 20 min. film exposure. (C) An identical blot was probed with [a-32P] dATP nick translated DNA. The film was exposed for 18 hours with intensifying screens. M=l/BstE ll.
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PHOTOPROBE® Reagents are nucleic acid labeling systems of choice for incorporating a label at multiple sites along the entire length of the nucleic acid. The nucleic acid labeling reaction does not destroy the original nucleic acid nor creates its copy. The integrity of the original sample is preserved. Single or double-stranded DNA, circular DNA, RNA, or PNA (peptide nucleic acid) can be labeled with the same reagents.
DNA/RNA labeling is based on aryl azide chemistry in which the reagent, when exposed to heat or light, becomes activated and incorporates into the nucleic acid without base specificity. The nucleic acid labeling reaction can be carried out using a mercury vapor bulb (sun lamp), a UV lamp which produces light in the 350 nm to 370 nm range, a halogen lamp, a heating block, or a thermal cycler providing the investigator with great flexibility in experimental design.
A distinct advantage of the simple setup in each of these labeling options is the ease and economy of scaling up.
PHOTOPROBE® Biotin incorporates biotin in one reaction step. PHOTOPROBE® (Long Arm) Biotin has an extra long linker arm and should be used if increased distance between the sample and the tag is needed. In a similar manner, PHOTOPROBE® Amine incorporates primary amines into nucleic acids. These amino groups can subsequently be used to attach haptens, affinity tags, or fluorochromes, or to immobilize nucleic acids to a solid matrix.
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The PHOTOPROBE® Biotin Labeling and Detection System provides the essential reagents for labeling DNA or RNA probes with biotin and detecting these probes on nitrocellulose and nylon membranes, or tissue sections with a chromogenic substrate.
Kit Contents:
The kit consists of the following:
PHOTOPROBE® (Long Arm) Biotin Labeling Kit
10x Casein Solution (250 ml concentrate)
Alkaline Phosphatase Streptavidin (1 ml)
BCIP/NBT Substrate Kit
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PHOTOPROBE Biotin System Video Tutorial
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Labeling DNA using the PHOTOPROBE Biotin system. Both light and heat activation of the PHOTOPROBE reagent are demonstrated in this step by step guide. |
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Dot Blot Assay Video Tutorial
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Step by step demonstration of the dot blot technique. |
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Biotin Quantitation using the QuantTag Biotin Kit
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Sensitive method of measuring biotin, free or bound to macromolecules using the QuantTag Kit. |
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