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Featuring:
Plus
quality reagents for:
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| Vector Fusion-Aid c-Myc Kit, (Agarose Anti-C-Myc)
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Creating fusion proteins using recombinant
DNA techniques is the preferred method to purify or detect
many proteins of interest.
Two proteins that are commonly fused to proteins of interest
are green fluorescent protein (GFP) and maltose binding
protein (MBP). Alternatively, proteins of interest are fused
to a specific peptide sequence rather than an entire protein.
This peptide sequence defines an epitope that can be readily
detected with a specific antibody. Two commonly used peptide
tags are derived from human influenza virus hemagglutinin
(HA) and c-Myc.
Fusion Protein Purification. The Fusion-Aid™ Kits are
designed to immunoprecipitate/isolate fusion proteins
containing the appropriate protein tag. The Fusion-Aid™
Kit has affinity-purified primary antibodies coupled directly
to agarose beads. In contrast to traditional methods, this
eliminates the contamination of the isolated proteins with
coprecipitating primary antibodies. The spin columns provided
with the kit also facilitate rapid immunoprecipitation and
maximum protein recovery.
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Vector® Fusion-Aid™ Kits contain the following:
0.5 ml agarose beads coupled with affinity purified antibody to the specific tag
5 spin columns
5 collection tubes
a detailed protocol
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