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Featuring:
Plus
quality reagents for:
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| DNA Molecular Weight Markers, Biotinylated
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This product is an electrophoresis standard consisting of 15
bands between 0.2 and 10 kilobase pairs. Bands are regularly
spaced so that sizes are easy to identify. Markers are supplied
ready-to-use containing loading dye so that electrophoresis can
be monitored.
The Biotinylated DNA Molecular Weight Marker (MB-1302) is
used when nucleic acids are to be transferred to a membrane
and later detected using an avidin or streptavidin-based
system.
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Ready-to-use marker (5 μl or 500 ng) was loaded on a
0.5% agarose gel. The gel was run in 1x TBE at 10 V/
cm, stained with ethidium bromide (left) or blotted
onto nylon membrane using standard methods.
Biotinylated DNA bands (right) were detected using
the UltraSNAP™ Kit (Alkaline Phosphatase Streptavidin
followed by DuoLuX™ Chemiluminescent Substrate).
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