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Featuring:
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DAB Peroxidase Substrate Kit, 3,3’-diaminobenzidine
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Tumor tissue section showing specific cytoplasmic cell staining (brown, Vector DAB) with Vector Hematoxylin QS (blue) counterstain.
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DAB (3, 3’-diaminobenzidine) produces a dark brown reaction
product and can be used for both immunohistochemical and
blotting applications. DAB is effective as a single label or as a
second color for multiple antigen labeling. Because of its heat-resistance, DAB can be used in IHC/
ISH double labeling applications. With the aid of imaging
systems and software, the spectral profile of DAB can be
distinguished from our other proprietary enzyme substrates
in applications where antigens are co-localized. Sections
stained with DAB can also be viewed by darkfield and electron
microscopy. Slides developed with DAB can be dehydrated,
cleared, and permanently mounted. The DAB reaction product
can be intensified with DAB Enhancing Solution (H-2200).
This kit contains stock solutions in convenient dropper bottles.
This kit includes nickel, providing the option of changing the
reaction product from brown to gray-black and increasing
sensitivity in blot applications. Slides developed with DAB/
Ni should be dehydrated, cleared, and permanently mounted.
This product can also be used on blots. This kit provides all of the necessary reagents to prepare about 300 ml of working solution. When stored at 4 ºC, this kit is stable for one year.
Product review on Biocompare.com.
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Vector DAB Substrate Kit Video Tutorial
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Development of the DAB substrate from the Vector DAB substrate kit. For additional instructional videos please see the Tutorials page under the Support link. |
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