Protocols

Selected Vector kit instructions and associated protocols are listed below in PDF format. If you are unable to obtain the desired information please contact Vector technical service or your local Vector distributor for further assistance.

 VECTASTAIN^® Kits

• VECTASTAIN^® Elite ABC-Peroxidase Kit (included in all PK-6100 series kits)
• VECTASTAIN^® Universal Elite ABC-Peroxidase Kit (PK-6200)
• R.T.U. VECTASTAIN^® Elite ABC-Peroxidase Reagent (PK-7100)
• R.T.U. VECTASTAIN^® Universal Elite ABC-Peroxidase Kit (PK-7200)
• VECTASTAIN^® ABC-Peroxidase Kit (included in all PK-4000 series kits)
• VECTASTAIN^® ABC-Alkaline Phosphatase Kit (included in all AK-5000 series kits)
• VECTASTAIN^® Universal ABC-Alkaline Phosphatase Kit (AK-5200)
• VECTASTAIN^® ABC-GO-Glucose Oxidase Kit (included in all OK-3000 series kits)
• VECTASTAIN^® Universal Quick Kit (PK-8800)
• R.T.U. VECTASTAIN^® Universal Quick Kit (PK-7800)
  

Supplementary Protocols for Vector Primary Antibodies

• High temperature antigen unmasking technique
• Trypsin digestion
• Pretreatment required for using the BrdU antibody on paraffin sections
• Immunohistochemical use of antibodies to Dystrophin
• Immunohistochemical use of antibodies to muscle disease related markers
• Use of antiviral antibodies in indirect immunofluorescence
• Use of Vector antibodies in direct flow cytometry

Supplemental Protocols for Vector^® Kits and Reagents

• Enzyme Immunoassays, Hybridoma Screening, and Western Blots
• BLOXALL™ Solution for Quenching Endogenous Peroxidase and Alkaline Phosphatase Activity
• Quenching of Endogenous Peroxidase Activity
• Recommended Dilutions and Buffers
• Troubleshooting Guide
• Enzyme Substrate Compatibility Chart

ImmPRESS™ Polymerized Reporter Enzyme Staining System

• ImmPRESS™-AP Anti-Rabbit Ig (alkaline phosphatase) (MP-5401)
• ImmPRESS™-AP Anti-Mouse Ig (alkaline phosphatase) (MP-5402)
• Vector^® Mouse on Mouse (M.O.M.™) ImmPRESS Peroxidase Polymer Immunodetection Kit (MP-2400)
• ImmPRESS™ Anti-Rabbit Ig (peroxidase) (MP-7401)
• ImmPRESS™ Anti-Mouse Ig (peroxidase) (MP-7402)
• ImmPRESS™ Anti-Mouse Ig, Rat-adsorbed (peroxidase) (MP-7422)
• ImmPRESS™ Anti-Rat Ig (peroxidase) (MP-7404)
• ImmPRESS™ Anti-Rat Ig, Mouse-adsorbed (peroxidase) (MP-7444)
• ImmPRESS™ Anti-Goat Ig (peroxidase) (MP-7405)
• ImmPRESS™ Universal Antibody Kit (peroxidase) (MP-7500)

Vector Mouse on Mouse (M.O.M.™) Immunodetection Kit

• Vector^® Mouse on Mouse (M.O.M.™) Elite Peroxidase Immunodetection Kit (PK-2200)
• Vector^® Mouse on Mouse (M.O.M.™) ImmPRESS Peroxidase Polymer Immunodetection Kit (MP-2400)
• Vector^® Mouse on Mouse (M.O.M.™) Fluorescein Immunodetection Kit (FMK-2201)
• Vector^® Mouse on Mouse (M.O.M.™) Basic Immunodetection Kit (BMK-2202)
• Multiple Immunofluorescent Staining Using Mouse Antibodies
• Troubleshooting mouse primaries on mouse tissue

Multiple Antigen Labeling in the Same Tissue Sections

Counterstains

• Vector^® Hematoxylin (H-3401)
• Vector^® Hematoxylin QS (H-3404)
• Vector^® Methyl Green (H-3402)
• Vector^® Nuclear Fast Red (H-3403)
• Counterstain/Substrate Compatibility Chart
  

Mounting Media

• VectaMount™Permanent Mounting Medium (H-5000)
• VectaMount™ AQ Aqueous Mounting Medium (H-5501)
• VECTASHIELD^® Mounting Media (H-1000)
• VECTASHIELD^® Mounting Media with DAPI (H-1200)
• VECTASHIELD^® Mounting Media with PI (H-1300)
• VECTASHIELD^® HardSet Mounting Media (H-1400)
• VECTASHIELD^® HardSet Mounting Media with DAPI (H-1500)
  

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Immunofluorescence

• Double Immunofluorescent Labeling

VECTABOND™ Reagent for Tissue Section Adhesion (SP-1800)

Antigen Unmasking Solution (H-3300, H-3301)

Avidin/Biotin Blocking Kit (SP-2001)

Streptavidin/Biotin Blocking Kit (SP-2002)

Enzyme Substrates

• Peroxidase
  • ImmPACT™ DAB Substrate Kit (SK-4105)
  • ImmPACT™ DAB EqV Substrate Kit (SK-4103)
  • ImmPACT™ AEC Substrate Kit (SK-4205)
  • ImmPACT™ AMEC Red Substrate Kit (SK-4285)
  • ImmPACT™ VIP Substrate Kit (SK-4605)
  • ImmPACT™ SG Substrate Kit (SK-4705)
  • ImmPACT™ NovaRED Substrate Kit (SK-4805)
  • DAB Substrate Kit (SK-4100)
  • DAB Enhancing Solution (H-2200)
  • Vector^® VIP Substrate Kit (SK-4600)
  • Vector^® SG Substrate Kit (SK-4700)
  • Vector^® NovaRED Substrate Kit (SK-4800)
  • AEC Substrate Kit (SK4200)
  • TMB Substrate Kit (SK-4400)
  • 4-CN Substrate Kit (SK-4300)
  • ABTS Substrate Kit (SK-4500)
  • DuoLuX™ Chemiluminescent/Fluorescent Substrate Kit for peroxidase (SK-6604)
• Alkaline Phosphatase
  • Vector^® Red Substrate Kit (SK-5100)
  • Vector^® Blue Substrate Kit (SK-5300)
  • Vector^® Black Substrate Kit (SK-5200)
  • BCIP/NBT Substrate Kit (SK-5400)
  • Levamisole Solution (SP-5000)
  • p-Nitrophenyl phosphate Substrate Kit (SK-5900)
  • DuoLuX™ Chemiluminescent/Fluorescent Substrate Kit for alkaline phosphatase (SK-6605)
• Glucose Oxidase
  • Glucose Oxidase Substrate Kit I (SK-3100)
  • Glucose Oxidase Substrate Kit II (SK-3200)
• Chemiluminescent/Fluorescent Substrate
  • DuoLuX™ Chemiluminescent/Fluorescent Substrate Kit for peroxidase (SK-6604)
  • DuoLuX™ Chemiluminescent/Fluorescent Substrate Kit for alkaline phosphatase (SK-6605)

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Blot Detection Systems

• WestVision™ Peroxidase Polymer Reagents for Western Blotting (WB-1000, WB-2000)
• VECTASTAIN^® ABC-AmP™ Western Blot Detection System (AK-6000 series kits)
• Vector^® UltraSNAP™ Detection System for nucleic acid blots (MB-6500)
• Troubleshooting Guide for VECTASTAIN® ABC-AmP™ Kits

In Situ Hybridization

• Fluorescence Detection of Biotin-Labeled ISH Probes
• Fluorescence Detection of Fluorescein-Labeled ISH Probes
• Chromogenic Detection of Biotin-Labeled ISH Probes
• Chromogenic Detection of Fluorescein-Labeled ISH Probes
• In Situ Hybridization Detection Systems (General guidelines)
• NicKit™ Probe Size Optimization Kit (MB-1905)
• Amplification of FISH using Biotinylated Anti-Avidin (BA-0300)

Protein Labeling Kits

• ProtOn™ Biotin Labeling Kit
• ProtOn™ Fluorescein Labeling Kit

Biotin Labeling Reagents

• Biotin (Long Arm) NHS (SP-1200)
• Biotin (Long Arm) NHS, water soluble (SP-1210)
• Biotin (Long Arm) Maleimide (SP-1501)
• Biotin (Long Arm) Hydrazide (SP-1100)

Biotin Quantitation Kit

• Quant*Tag Biotin Kit (BDK-2000)

Thiol-Reactive Labeling Reagents

• Biotin Maleimide (SP-1501)
• Dinitrophenyl Maleimide (SP-1503)
• Fluorescein Maleimide (SP-1502)
• Fucose Maleimide (SP-1504)
• Texas Red Maleimide (SP-1505)

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Molecular Biology Reagents

• 5' EndTag Labeling System (MB-9001)
• 3' EndTag Labeling System (MB-9002)
• PHOTOPROBE^® Biotin (SP-1000, SP-1010, SP-1020, SPK-1906)
• PHOTOPROBE^® Amine (SP-1070)
• FastTag^® Basic Kit (MB-8000)
• NicKit™ Probe Size Optimization Kit (MB-1905)
• In Situ Hybridization Detection Systems
• Amplification of FISH using Biotinylated Anti-Avidin (BA-0300)
• Detection of Labeled Nucleic Acid Probes on Blots
• Spin Column Chromatography
• Modified FastTag^® labeling protocol for PCR Primers
  
• Irreversible Affinity Binding Matrices
  • VECTREX^® Avidin D (A-2020)
• Reversible Affinity Binding Matrices
  • VECTREX^® Avidin DLA (MB-2022)
  • VECTREX^® AAL (MB-1397)

Fusion Protein Reagents